Quantitative Proteomic Analysis of Human Seminal Plasma from Normozoospermic and Asthenozoospermic Individuals
Joint Authors
Wu, Yibo
Yuan, Yan
Chen, Ling
Wang, Min
Yang, Yong
Wang, Yingnan
Quan, Chao
Chen, Daozhen
Chen, Ying
Huang, Xiaoyan
Zhou, Tao
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Seminal plasma is a complex mixture of secretions from various glands in the male genital tract.
Compared to sperm cells, it contains important proteins that are both directly and indirectly associated with sperm motility.
Here, we constructed quantitative proteomes of human seminal plasma from three normozoospermic and asthenozoospermic individuals.
A total of 524 proteins were identified, and 366 of them were found to be quantified in all six samples.
We first investigated the absolute expression features of these proteins and found that the variations of protein identification among different samples and other published datasets were mainly due to some lowly expressed proteins.
By integration of various proteomic datasets and bioinformatics databases, we comprehensively annotated the biological functions, physiological originations, and disease associations of these proteins.
We found that our dataset could benefit the studies of both male infertility and other male diseases.
Finally, based on the relative expression values determined by chemical labeling, we identified a total of 29 differentially expressed proteins, which could be used as candidate targets for studying the molecular bases of sperm motility or developing precise diagnostic biomarkers of asthenozoospermia.
We further successfully verified the expression trends of four representative proteins by Western blotting.
Compared to a previous dataset based on label-free quantification, our results showed that most of the important proteins could be identified in the sample collected only once for each individual, providing the bases for personalized examination of seminal plasma proteins in clinic.
American Psychological Association (APA)
Wu, Yibo& Yuan, Yan& Chen, Ling& Wang, Min& Yang, Yong& Wang, Yingnan…[et al.]. 2019. Quantitative Proteomic Analysis of Human Seminal Plasma from Normozoospermic and Asthenozoospermic Individuals. BioMed Research International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1124049
Modern Language Association (MLA)
Wu, Yibo…[et al.]. Quantitative Proteomic Analysis of Human Seminal Plasma from Normozoospermic and Asthenozoospermic Individuals. BioMed Research International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1124049
American Medical Association (AMA)
Wu, Yibo& Yuan, Yan& Chen, Ling& Wang, Min& Yang, Yong& Wang, Yingnan…[et al.]. Quantitative Proteomic Analysis of Human Seminal Plasma from Normozoospermic and Asthenozoospermic Individuals. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1124049
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124049