Vitamin D Regulates the Expressions of AQP-1 and AQP-4 in Mice Kidneys

الملخص EN

Aim.

Vitamin D plays an important role in water and salt homeostasis.

The aim of our study was to investigate the underlying relationship of Vitamin D and Aquaporins (AQP).

Methods.

The behaviors of 1α (OH)-ase knockout mice and wild type mice were observed before analysis.

The ICR mice were treated with vehicle or paricalcitol, a vitamin D analogue, followed by animals receiving a standard diet and free access to drinking water either with aliskiren (renin blocker; 37.5 mg aliskiren in 100 ml water), or telmisartan (a angiotensin II type I receptor blocker; 40 mg telmisartan in 100 ml water) a week before study.

The expressions of AQP-1, AQP-4, and renin in mice kidneys were detected by western bolting, immunohistochemistry, and immunofluorescence.

Results.

Diuresis and polydipsia were observed in 1α (OH)-ase knockout mice, and a decreased water intake and urine output in ICR mice was observed after paricalcitol treatment.

Compared with wild type, the AQP-1 expressions were increased in renal papilla and AQP-4 expressions were decreased in renal proximal tubule of 1α(OH) ase knockout mice.

In addition, AQP-1 was decreased in renal papilla and AQP-4 expressions were increased in proximal tubule by suppressing renin activity or supplement of Vitamin D analogue.

After injecting renin into the lateral ventricle of the 1α(OH)ase knockout mice, the renin expression level was decreased in the kidney, followed by the decrease of AQP-1 in renal papilla and increase of AQP-4 in proximal tubule.

Conclusions.

Overall, Vitamin D and renin inhibitors have synergistic effects in regulating water channels in mice kidneys.