Vitamin D Regulates the Expressions of AQP-1 and AQP-4 in Mice Kidneys
Joint Authors
Fu, Yu
Zhu, Jiajun
Zhang, Yalin
Liu, Zuwang
Su, Han
Kong, Juan
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Aim.
Vitamin D plays an important role in water and salt homeostasis.
The aim of our study was to investigate the underlying relationship of Vitamin D and Aquaporins (AQP).
Methods.
The behaviors of 1α (OH)-ase knockout mice and wild type mice were observed before analysis.
The ICR mice were treated with vehicle or paricalcitol, a vitamin D analogue, followed by animals receiving a standard diet and free access to drinking water either with aliskiren (renin blocker; 37.5 mg aliskiren in 100 ml water), or telmisartan (a angiotensin II type I receptor blocker; 40 mg telmisartan in 100 ml water) a week before study.
The expressions of AQP-1, AQP-4, and renin in mice kidneys were detected by western bolting, immunohistochemistry, and immunofluorescence.
Results.
Diuresis and polydipsia were observed in 1α (OH)-ase knockout mice, and a decreased water intake and urine output in ICR mice was observed after paricalcitol treatment.
Compared with wild type, the AQP-1 expressions were increased in renal papilla and AQP-4 expressions were decreased in renal proximal tubule of 1α(OH) ase knockout mice.
In addition, AQP-1 was decreased in renal papilla and AQP-4 expressions were increased in proximal tubule by suppressing renin activity or supplement of Vitamin D analogue.
After injecting renin into the lateral ventricle of the 1α(OH)ase knockout mice, the renin expression level was decreased in the kidney, followed by the decrease of AQP-1 in renal papilla and increase of AQP-4 in proximal tubule.
Conclusions.
Overall, Vitamin D and renin inhibitors have synergistic effects in regulating water channels in mice kidneys.
American Psychological Association (APA)
Fu, Yu& Zhu, Jiajun& Zhang, Yalin& Liu, Zuwang& Su, Han& Kong, Juan. 2019. Vitamin D Regulates the Expressions of AQP-1 and AQP-4 in Mice Kidneys. BioMed Research International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1124273
Modern Language Association (MLA)
Fu, Yu…[et al.]. Vitamin D Regulates the Expressions of AQP-1 and AQP-4 in Mice Kidneys. BioMed Research International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1124273
American Medical Association (AMA)
Fu, Yu& Zhu, Jiajun& Zhang, Yalin& Liu, Zuwang& Su, Han& Kong, Juan. Vitamin D Regulates the Expressions of AQP-1 and AQP-4 in Mice Kidneys. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1124273
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124273