Enhanced Production of Polymyxin E in Paenibacillus polymyxa by Replacement of Glucose by Starch
المؤلفون المشاركون
Yu, Zhiliang
Qiu, Juanping
Sun, Zhongqi
Yin, Jianhua
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-10-14
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Polymyxin E or colistin, produced by Paenibacillus polymyxa, is an important antibiotic against Gram-negative pathogens.
The objective of this study is to evaluate the effect of starch in fermentation medium on colistin biosynthesis in P.
polymyxa.
The results indicated that replacement of glucose by starch stimulated colistin production and biosynthesis rate.
Overall, the stimulation extent was starch concentration-dependent.
As expected, addition of starch induced the expression of amyE encoding amylase and increased amylase activity in fermentation solution.
Additionally, replacement of glucose by starch resulted in residue reducing sugar and pH of fermentation mixture low relative to glucose as the sole sugar source.
At the molecular level, it was found that replacement of glucose by starch has enhanced the relative expression level of ccpA encoding catabolite control protein A.
Therefore, the repression of starch utilization by glucose could be probably relieved.
In addition, use of starch stimulated the expression of regulatory gene spo0A but repressed the expression of another regulatory gene abrB.
As a result, the expression of genes directly involved in colistin biosynthesis and secretion increased, indicating that at the transcriptional level spo0A and abrB played opposite roles in regulating colistin biosynthesis in P.
polymyxa.
Taken together, our data demonstrated that starch instead of glucose can promote colistin production probably by affecting the expression of colistin biosynthesis-related genes, as well as reducing the repression of glucose to a secondary metabolic product.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yu, Zhiliang& Sun, Zhongqi& Yin, Jianhua& Qiu, Juanping. 2018. Enhanced Production of Polymyxin E in Paenibacillus polymyxa by Replacement of Glucose by Starch. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124768
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yu, Zhiliang…[et al.]. Enhanced Production of Polymyxin E in Paenibacillus polymyxa by Replacement of Glucose by Starch. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1124768
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yu, Zhiliang& Sun, Zhongqi& Yin, Jianhua& Qiu, Juanping. Enhanced Production of Polymyxin E in Paenibacillus polymyxa by Replacement of Glucose by Starch. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124768
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1124768
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر