Enhanced Production of Polymyxin E in Paenibacillus polymyxa by Replacement of Glucose by Starch
Joint Authors
Yu, Zhiliang
Qiu, Juanping
Sun, Zhongqi
Yin, Jianhua
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Polymyxin E or colistin, produced by Paenibacillus polymyxa, is an important antibiotic against Gram-negative pathogens.
The objective of this study is to evaluate the effect of starch in fermentation medium on colistin biosynthesis in P.
polymyxa.
The results indicated that replacement of glucose by starch stimulated colistin production and biosynthesis rate.
Overall, the stimulation extent was starch concentration-dependent.
As expected, addition of starch induced the expression of amyE encoding amylase and increased amylase activity in fermentation solution.
Additionally, replacement of glucose by starch resulted in residue reducing sugar and pH of fermentation mixture low relative to glucose as the sole sugar source.
At the molecular level, it was found that replacement of glucose by starch has enhanced the relative expression level of ccpA encoding catabolite control protein A.
Therefore, the repression of starch utilization by glucose could be probably relieved.
In addition, use of starch stimulated the expression of regulatory gene spo0A but repressed the expression of another regulatory gene abrB.
As a result, the expression of genes directly involved in colistin biosynthesis and secretion increased, indicating that at the transcriptional level spo0A and abrB played opposite roles in regulating colistin biosynthesis in P.
polymyxa.
Taken together, our data demonstrated that starch instead of glucose can promote colistin production probably by affecting the expression of colistin biosynthesis-related genes, as well as reducing the repression of glucose to a secondary metabolic product.
American Psychological Association (APA)
Yu, Zhiliang& Sun, Zhongqi& Yin, Jianhua& Qiu, Juanping. 2018. Enhanced Production of Polymyxin E in Paenibacillus polymyxa by Replacement of Glucose by Starch. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124768
Modern Language Association (MLA)
Yu, Zhiliang…[et al.]. Enhanced Production of Polymyxin E in Paenibacillus polymyxa by Replacement of Glucose by Starch. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1124768
American Medical Association (AMA)
Yu, Zhiliang& Sun, Zhongqi& Yin, Jianhua& Qiu, Juanping. Enhanced Production of Polymyxin E in Paenibacillus polymyxa by Replacement of Glucose by Starch. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124768
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124768