Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property
المؤلفون المشاركون
Lu, Xiang-yang
Li, Tao
Zhu, Jihe
Deng, Fangming
Wu, Weiguo
Zheng, Zhibing
Lv, Chenghao
Li, Yong
Xiang, Wei
Qin, Si
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-07
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Myricetin has been reported as a promising chemopreventive compound with multiple biofunctions.
To evaluate its influence on gene expressions in genome-wide set and further investigate its anti-inflammatory property, the present study performed Gene Ontology and Ingenuity Pathway Analysis (IPA) to describe the basic gene expression characteristics by myricetin treatment in HepG2 cells, confirmed its multi-biofunction by real-time fluorescent quantitative PCR (RT-qPCR), and further verified its anti-inflammatory property by Western blotting and bio-plex-based cytokines assay.
The IPA data showed that 337 gene expressions (48% of the top molecules) are disturbed over 2-fold, and the most possible biofunctions of myricetin are the effect on “cardiovascular disease, metabolic disease, and lipid metabolism,” via regulation of 28 molecules with statistic score of 46.
RT-qPCR data confirmed the accuracy of microarray data, and cytokines assay results indicated that 6 of the total 27 inflammatory cytokine secretions were significantly inhibited by myricetin pretreatment, including TNF-α, IFN-γ, IL-1α, IL-1β, IL-2, and IL-6.
The present study is the first time to elucidate the multi-function of myricetin in genome-wide set by IPA analysis and verify its anti-inflammatory property by proteomics of cytokines assay.
Therefore, these results enrich the comprehensive bioactivities of myricetin and reveal that myricetin has powerful anti-inflammatory property, which provides encouragement for in vivo studies to verify its possible health benefits.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Tao& Zhu, Jihe& Deng, Fangming& Wu, Weiguo& Zheng, Zhibing& Lv, Chenghao…[et al.]. 2019. Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property. BioMed Research International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1124814
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Tao…[et al.]. Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property. BioMed Research International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1124814
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Tao& Zhu, Jihe& Deng, Fangming& Wu, Weiguo& Zheng, Zhibing& Lv, Chenghao…[et al.]. Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1124814
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1124814
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر