Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property
Joint Authors
Lu, Xiang-yang
Li, Tao
Zhu, Jihe
Deng, Fangming
Wu, Weiguo
Zheng, Zhibing
Lv, Chenghao
Li, Yong
Xiang, Wei
Qin, Si
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-07
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Myricetin has been reported as a promising chemopreventive compound with multiple biofunctions.
To evaluate its influence on gene expressions in genome-wide set and further investigate its anti-inflammatory property, the present study performed Gene Ontology and Ingenuity Pathway Analysis (IPA) to describe the basic gene expression characteristics by myricetin treatment in HepG2 cells, confirmed its multi-biofunction by real-time fluorescent quantitative PCR (RT-qPCR), and further verified its anti-inflammatory property by Western blotting and bio-plex-based cytokines assay.
The IPA data showed that 337 gene expressions (48% of the top molecules) are disturbed over 2-fold, and the most possible biofunctions of myricetin are the effect on “cardiovascular disease, metabolic disease, and lipid metabolism,” via regulation of 28 molecules with statistic score of 46.
RT-qPCR data confirmed the accuracy of microarray data, and cytokines assay results indicated that 6 of the total 27 inflammatory cytokine secretions were significantly inhibited by myricetin pretreatment, including TNF-α, IFN-γ, IL-1α, IL-1β, IL-2, and IL-6.
The present study is the first time to elucidate the multi-function of myricetin in genome-wide set by IPA analysis and verify its anti-inflammatory property by proteomics of cytokines assay.
Therefore, these results enrich the comprehensive bioactivities of myricetin and reveal that myricetin has powerful anti-inflammatory property, which provides encouragement for in vivo studies to verify its possible health benefits.
American Psychological Association (APA)
Li, Tao& Zhu, Jihe& Deng, Fangming& Wu, Weiguo& Zheng, Zhibing& Lv, Chenghao…[et al.]. 2019. Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property. BioMed Research International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1124814
Modern Language Association (MLA)
Li, Tao…[et al.]. Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property. BioMed Research International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1124814
American Medical Association (AMA)
Li, Tao& Zhu, Jihe& Deng, Fangming& Wu, Weiguo& Zheng, Zhibing& Lv, Chenghao…[et al.]. Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1124814
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124814