RNA Sequencing for Gene Expression Profiles in a Rat Model of Middle Cerebral Artery Occlusion
المؤلفون المشاركون
Han, Lan
Xu, Fan
Peng, Daiyin
Duan, Xianchun
Gan, Jianghua
Li, Lili
Peng, Can
Bao, Qiuyu
Xiao, Ling
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-11-06
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Gene expression regulatory mechanisms in models of middle cerebral artery occlusion (MCAO) have been assessed in some studies, but questions remain.
The discovery of differentially expressed genes (DEGs) between MCAO and control rats analyzed by next-generation RNA sequencing is of particular interest.
These DEGs may help guide the clinical diagnosis of stroke and facilitate selection of the optimal treatment strategy.
Twenty rats were equally divided into control and MCAO groups.
Three rats from each group were randomly selected for RNA sequencing analysis.
Sequence reads were obtained from an Illumina HiSeq2500 platform and mapped onto the rat reference genome RN6 using Hisat2.
We identified 1,007 significantly DEGs with p<0.05, including 785 upregulated (fold change [FC]>2) and 222 downregulated (FC<0.5) DEGs, in brain tissue from MCAO rats compared with that from control rats, and numerous immune response genes were identified.
Gene ontology (GO) analysis revealed that the majority of the most enriched and meaningful biological process terms were mainly involved in immune response, inflammatory response, cell activation, leukocyte migration, cell adhesion, response to external stimulus, cell migration, and response to wounding.
Also enriched were immune-related pathways and neural-related pathways.
Similar to GO molecular function terms, the enriched terms were mainly related to cytokine receptor activity.
Six DEGs were verified by quantitative real-time polymerase chain reaction (qRT-PCR).
Protein-protein interaction analysis of these hits revealed that MCAO affects complement and coagulation cascades and chemokine signaling pathway.
Our study identified novel biomarkers that could help further elucidate MCAO mechanisms and processes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Duan, Xianchun& Gan, Jianghua& Xu, Fan& Li, Lili& Han, Lan& Peng, Can…[et al.]. 2018. RNA Sequencing for Gene Expression Profiles in a Rat Model of Middle Cerebral Artery Occlusion. BioMed Research International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1125071
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Duan, Xianchun…[et al.]. RNA Sequencing for Gene Expression Profiles in a Rat Model of Middle Cerebral Artery Occlusion. BioMed Research International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1125071
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Duan, Xianchun& Gan, Jianghua& Xu, Fan& Li, Lili& Han, Lan& Peng, Can…[et al.]. RNA Sequencing for Gene Expression Profiles in a Rat Model of Middle Cerebral Artery Occlusion. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1125071
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125071
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر