RNA Sequencing for Gene Expression Profiles in a Rat Model of Middle Cerebral Artery Occlusion
Joint Authors
Han, Lan
Xu, Fan
Peng, Daiyin
Duan, Xianchun
Gan, Jianghua
Li, Lili
Peng, Can
Bao, Qiuyu
Xiao, Ling
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-06
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Gene expression regulatory mechanisms in models of middle cerebral artery occlusion (MCAO) have been assessed in some studies, but questions remain.
The discovery of differentially expressed genes (DEGs) between MCAO and control rats analyzed by next-generation RNA sequencing is of particular interest.
These DEGs may help guide the clinical diagnosis of stroke and facilitate selection of the optimal treatment strategy.
Twenty rats were equally divided into control and MCAO groups.
Three rats from each group were randomly selected for RNA sequencing analysis.
Sequence reads were obtained from an Illumina HiSeq2500 platform and mapped onto the rat reference genome RN6 using Hisat2.
We identified 1,007 significantly DEGs with p<0.05, including 785 upregulated (fold change [FC]>2) and 222 downregulated (FC<0.5) DEGs, in brain tissue from MCAO rats compared with that from control rats, and numerous immune response genes were identified.
Gene ontology (GO) analysis revealed that the majority of the most enriched and meaningful biological process terms were mainly involved in immune response, inflammatory response, cell activation, leukocyte migration, cell adhesion, response to external stimulus, cell migration, and response to wounding.
Also enriched were immune-related pathways and neural-related pathways.
Similar to GO molecular function terms, the enriched terms were mainly related to cytokine receptor activity.
Six DEGs were verified by quantitative real-time polymerase chain reaction (qRT-PCR).
Protein-protein interaction analysis of these hits revealed that MCAO affects complement and coagulation cascades and chemokine signaling pathway.
Our study identified novel biomarkers that could help further elucidate MCAO mechanisms and processes.
American Psychological Association (APA)
Duan, Xianchun& Gan, Jianghua& Xu, Fan& Li, Lili& Han, Lan& Peng, Can…[et al.]. 2018. RNA Sequencing for Gene Expression Profiles in a Rat Model of Middle Cerebral Artery Occlusion. BioMed Research International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1125071
Modern Language Association (MLA)
Duan, Xianchun…[et al.]. RNA Sequencing for Gene Expression Profiles in a Rat Model of Middle Cerebral Artery Occlusion. BioMed Research International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1125071
American Medical Association (AMA)
Duan, Xianchun& Gan, Jianghua& Xu, Fan& Li, Lili& Han, Lan& Peng, Can…[et al.]. RNA Sequencing for Gene Expression Profiles in a Rat Model of Middle Cerebral Artery Occlusion. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1125071
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125071