Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction
المؤلف
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-22
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
This study explores determining the sex of humans from blood stains taken from different surfaces and compares the time course of detection with the conventional PCR, Conventional Loop Mediated Isothermal Amplification (LAMP), and LAMP-Lateral Flow Dipstick (LFD).
For the DNA templates, 7 male and 7 female blood stained samples were extracted and added to LAMP and PCR reaction solution to amplify the SRY gene.
The DNA samples were extracted from the following blood stained materials: cloth, wood, clay, and tile.
Then, the samples were stored at room temperature for 1, 7, 30, and 60 day(s).
After the DNA amplification, the gel electrophoresis process was applied to detect LAMP product.
The LFD was combined with the LAMP to detect LAMP product on the male cloth samples.
For the male samples, the time course of detection on the first and seventh days indicated positive for both LAMP and PCR products on all the surfaces while no DNA amplification was found on any of the female samples.
On day 30, positive LAMP product was still found on all the male samples.
However, it had faded on the tiles.
Moreover, all the male samples, which had tested positive for PCR product, were blurred and unclear.
On day 60, LAMP product was still found on all the male samples.
Conversely, the PCR method resulted in no bands showing for any of the male samples.
However, the LAMP-LFD method detected product on all the male samples of cloth.
The results show that the LAMP is an effective, practical, and reliable molecular-biological method.
Moreover, the LFD can increase the efficiency and sensitivity of the LAMP, making it more suitable for field studies because gel electrophoresis apparatus is not required.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kanchanaphum, Panan. 2018. Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125553
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kanchanaphum, Panan. Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1125553
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kanchanaphum, Panan. Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125553
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125553
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر