Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction
Author
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
This study explores determining the sex of humans from blood stains taken from different surfaces and compares the time course of detection with the conventional PCR, Conventional Loop Mediated Isothermal Amplification (LAMP), and LAMP-Lateral Flow Dipstick (LFD).
For the DNA templates, 7 male and 7 female blood stained samples were extracted and added to LAMP and PCR reaction solution to amplify the SRY gene.
The DNA samples were extracted from the following blood stained materials: cloth, wood, clay, and tile.
Then, the samples were stored at room temperature for 1, 7, 30, and 60 day(s).
After the DNA amplification, the gel electrophoresis process was applied to detect LAMP product.
The LFD was combined with the LAMP to detect LAMP product on the male cloth samples.
For the male samples, the time course of detection on the first and seventh days indicated positive for both LAMP and PCR products on all the surfaces while no DNA amplification was found on any of the female samples.
On day 30, positive LAMP product was still found on all the male samples.
However, it had faded on the tiles.
Moreover, all the male samples, which had tested positive for PCR product, were blurred and unclear.
On day 60, LAMP product was still found on all the male samples.
Conversely, the PCR method resulted in no bands showing for any of the male samples.
However, the LAMP-LFD method detected product on all the male samples of cloth.
The results show that the LAMP is an effective, practical, and reliable molecular-biological method.
Moreover, the LFD can increase the efficiency and sensitivity of the LAMP, making it more suitable for field studies because gel electrophoresis apparatus is not required.
American Psychological Association (APA)
Kanchanaphum, Panan. 2018. Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125553
Modern Language Association (MLA)
Kanchanaphum, Panan. Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1125553
American Medical Association (AMA)
Kanchanaphum, Panan. Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125553
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125553