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Downregulation of miR-224-5p Promotes Migration and Proliferation in Human Dental Pulp Stem Cells
المؤلفون المشاركون
Ke, Zhihong
Qiu, Zailing
Xiao, Tingting
Zeng, Jianchai
Zou, Luning
Lin, Xuemei
Hu, Xuegang
Lin, Shihan
Lv, Hongbing
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-07-18
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Introduction.
Pulp regeneration, as a treatment for pulp necrosis, has significant advantages over root canal therapy for the preservation of living pulp.
To date, research on pulp regeneration has mainly focused on the transplantation of pulp stem cells into the root canal, but there is still a lack of research on the migration of pulp cells into the root canal via cell homing.
Stem cells from the apical tooth papilla (SCAP) are recognized as multidirectional stem cells, but these cells are difficult to obtain.
MicroRNAs are small noncoding RNAs that play crucial roles in regulating normal and pathologic functions.
We hypothesized that some types of microRNAs might improve the migration and proliferation function of dental pulp stem cells (DPSCs), which are easily obtained in clinical practice, and as a result, DPSCs might replace SCAP and provide valuable information for regenerative endodontics.
Methods.
Magnetic activated cell sorting of DPSCs and SCAP was performed.
Next-generation sequencing was performed to examine DPSCs and SCAP miRNAs expression and to identify the most significant differentially expressed miRNA.
CCK-8 and transwell assays were used to determine the impact of this miRNA on DPSCs proliferation and migration.
Results.
The most significant differentially expressed miRNA between DPSCs and SCAP was miR-224-5p.
Downregulating miR-224-5p promoted DPSCs proliferation and migration; the opposite results were observed when miR-224-5p was upregulated.
Conclusion.
MiR-224-5p promotes proliferation and migration in DPSCs, a finding that is of great significance for further exploring the role of dental pulp stem cells in regenerative endodontics.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ke, Zhihong& Qiu, Zailing& Xiao, Tingting& Zeng, Jianchai& Zou, Luning& Lin, Xuemei…[et al.]. 2019. Downregulation of miR-224-5p Promotes Migration and Proliferation in Human Dental Pulp Stem Cells. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125577
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ke, Zhihong…[et al.]. Downregulation of miR-224-5p Promotes Migration and Proliferation in Human Dental Pulp Stem Cells. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1125577
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ke, Zhihong& Qiu, Zailing& Xiao, Tingting& Zeng, Jianchai& Zou, Luning& Lin, Xuemei…[et al.]. Downregulation of miR-224-5p Promotes Migration and Proliferation in Human Dental Pulp Stem Cells. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125577
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125577
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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