Downregulation of miR-224-5p Promotes Migration and Proliferation in Human Dental Pulp Stem Cells
Joint Authors
Ke, Zhihong
Qiu, Zailing
Xiao, Tingting
Zeng, Jianchai
Zou, Luning
Lin, Xuemei
Hu, Xuegang
Lin, Shihan
Lv, Hongbing
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Introduction.
Pulp regeneration, as a treatment for pulp necrosis, has significant advantages over root canal therapy for the preservation of living pulp.
To date, research on pulp regeneration has mainly focused on the transplantation of pulp stem cells into the root canal, but there is still a lack of research on the migration of pulp cells into the root canal via cell homing.
Stem cells from the apical tooth papilla (SCAP) are recognized as multidirectional stem cells, but these cells are difficult to obtain.
MicroRNAs are small noncoding RNAs that play crucial roles in regulating normal and pathologic functions.
We hypothesized that some types of microRNAs might improve the migration and proliferation function of dental pulp stem cells (DPSCs), which are easily obtained in clinical practice, and as a result, DPSCs might replace SCAP and provide valuable information for regenerative endodontics.
Methods.
Magnetic activated cell sorting of DPSCs and SCAP was performed.
Next-generation sequencing was performed to examine DPSCs and SCAP miRNAs expression and to identify the most significant differentially expressed miRNA.
CCK-8 and transwell assays were used to determine the impact of this miRNA on DPSCs proliferation and migration.
Results.
The most significant differentially expressed miRNA between DPSCs and SCAP was miR-224-5p.
Downregulating miR-224-5p promoted DPSCs proliferation and migration; the opposite results were observed when miR-224-5p was upregulated.
Conclusion.
MiR-224-5p promotes proliferation and migration in DPSCs, a finding that is of great significance for further exploring the role of dental pulp stem cells in regenerative endodontics.
American Psychological Association (APA)
Ke, Zhihong& Qiu, Zailing& Xiao, Tingting& Zeng, Jianchai& Zou, Luning& Lin, Xuemei…[et al.]. 2019. Downregulation of miR-224-5p Promotes Migration and Proliferation in Human Dental Pulp Stem Cells. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125577
Modern Language Association (MLA)
Ke, Zhihong…[et al.]. Downregulation of miR-224-5p Promotes Migration and Proliferation in Human Dental Pulp Stem Cells. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1125577
American Medical Association (AMA)
Ke, Zhihong& Qiu, Zailing& Xiao, Tingting& Zeng, Jianchai& Zou, Luning& Lin, Xuemei…[et al.]. Downregulation of miR-224-5p Promotes Migration and Proliferation in Human Dental Pulp Stem Cells. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125577
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125577