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Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods
المؤلفون المشاركون
Saadeldin, Islam M.
Swelum, Ayman Abdel-Aziz
Abdelazim, Aaser M.
Almadaly, Essam A.
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-02-21
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Recent studies showed the modulatory effect of kisspeptin (KP) on calcium waves through the cell membrane and inside the cell.
Spermatozoon can induce similar ooplasmic calcium oscillations at fertilization to trigger meiosis II.
Here, we evaluated the effect of KP supplementation with 6-dimethylaminopurine (6-DMAP) for 4 h on embryonic development after oocyte activation with single electric pulse, 5 µM ionomycin, or 8% ethanol.
Compared to control nonsupplemented groups, KP significantly improved embryo developmental competence electric- and ethanol-activated oocytes in terms of cleavage (75.3% and 58.6% versus 64% and 48%, respectively, p<0.05) and blastocyst development (31.3% and 10% versus 19.3% and 4%, respectively, p<0.05).
MOS expression was increased in electrically activated oocytes in presence of KP while it significantly reduced CCNB1 expression.
In ionomycin treated group, both MOS and CCNB1 showed significant increase with no difference between KP and control groups.
In ethanol-treated group, KP significantly reduced CCNB1 but no effect was observed on MOS expression.
The early alterations in MOS and CCNB1 mRNA transcripts caused by KP may explain the significant differences in the developmental competence between the experimental groups.
Kisspeptin supplementation may be adopted in protocols for porcine oocyte activation through electric current and ethanol to improve embryonic developmental competence.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Saadeldin, Islam M.& Swelum, Ayman Abdel-Aziz& Abdelazim, Aaser M.& Almadaly, Essam A.. 2018. Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1126010
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Saadeldin, Islam M.…[et al.]. Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1126010
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Saadeldin, Islam M.& Swelum, Ayman Abdel-Aziz& Abdelazim, Aaser M.& Almadaly, Essam A.. Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1126010
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126010
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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