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Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods
Joint Authors
Saadeldin, Islam M.
Swelum, Ayman Abdel-Aziz
Abdelazim, Aaser M.
Almadaly, Essam A.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Recent studies showed the modulatory effect of kisspeptin (KP) on calcium waves through the cell membrane and inside the cell.
Spermatozoon can induce similar ooplasmic calcium oscillations at fertilization to trigger meiosis II.
Here, we evaluated the effect of KP supplementation with 6-dimethylaminopurine (6-DMAP) for 4 h on embryonic development after oocyte activation with single electric pulse, 5 µM ionomycin, or 8% ethanol.
Compared to control nonsupplemented groups, KP significantly improved embryo developmental competence electric- and ethanol-activated oocytes in terms of cleavage (75.3% and 58.6% versus 64% and 48%, respectively, p<0.05) and blastocyst development (31.3% and 10% versus 19.3% and 4%, respectively, p<0.05).
MOS expression was increased in electrically activated oocytes in presence of KP while it significantly reduced CCNB1 expression.
In ionomycin treated group, both MOS and CCNB1 showed significant increase with no difference between KP and control groups.
In ethanol-treated group, KP significantly reduced CCNB1 but no effect was observed on MOS expression.
The early alterations in MOS and CCNB1 mRNA transcripts caused by KP may explain the significant differences in the developmental competence between the experimental groups.
Kisspeptin supplementation may be adopted in protocols for porcine oocyte activation through electric current and ethanol to improve embryonic developmental competence.
American Psychological Association (APA)
Saadeldin, Islam M.& Swelum, Ayman Abdel-Aziz& Abdelazim, Aaser M.& Almadaly, Essam A.. 2018. Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1126010
Modern Language Association (MLA)
Saadeldin, Islam M.…[et al.]. Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1126010
American Medical Association (AMA)
Saadeldin, Islam M.& Swelum, Ayman Abdel-Aziz& Abdelazim, Aaser M.& Almadaly, Essam A.. Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1126010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126010