Polyphenol Polymerization by an Alternative Oxidative Microbial Enzyme and Characterization of the Biological Activity of Oligomers
المؤلفون المشاركون
Sabatini, Valentina
Di Gennaro, Patrizia
Sello, Guido
Fallarini, Silvia
Pagliarin, Roberto
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-23
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The recombinant catalase-peroxidase HPI from E.
coli was used as an alternative enzyme in polymerization reactions for the production of (−) epicatechin oligomers and their biological activity was characterized.
The enzyme was prepared in two forms: a purified and an immobilized form.
Both were tested for their activity in oxidative polymerization reactions, and their stability and reusability were assessed.
The polymerization reactions were followed by SEC-HPLC analyses, and the substrate was completely converted into one or more polymerization products depending on the reactions conditions.
Results showed that the utilized conditions allowed for the isolation of some oligomers of different molecular weight: the oligomers containing 6 and 7 units of epicatechin substrate are the heaviest ones.
Epicatechin was also used in reactions catalyzed by HRP in the same reaction conditions for comparison.
In addition, one selected oligomer obtained by HPI enzyme catalysis was shown to act as in vitro inhibitor of tumor cell growth, like one oligomer deriving from epicatechin by HRP catalysis.
These data confirm that epicatechin oligomeric form is more effective than its monomer in biological activity and suggest the use of HPI as an alternative enzyme in reactions for the production of epicatechin oligomers.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Di Gennaro, Patrizia& Sabatini, Valentina& Fallarini, Silvia& Pagliarin, Roberto& Sello, Guido. 2018. Polyphenol Polymerization by an Alternative Oxidative Microbial Enzyme and Characterization of the Biological Activity of Oligomers. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126137
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Di Gennaro, Patrizia…[et al.]. Polyphenol Polymerization by an Alternative Oxidative Microbial Enzyme and Characterization of the Biological Activity of Oligomers. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1126137
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Di Gennaro, Patrizia& Sabatini, Valentina& Fallarini, Silvia& Pagliarin, Roberto& Sello, Guido. Polyphenol Polymerization by an Alternative Oxidative Microbial Enzyme and Characterization of the Biological Activity of Oligomers. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126137
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126137
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر