Polyphenol Polymerization by an Alternative Oxidative Microbial Enzyme and Characterization of the Biological Activity of Oligomers
Joint Authors
Sabatini, Valentina
Di Gennaro, Patrizia
Sello, Guido
Fallarini, Silvia
Pagliarin, Roberto
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The recombinant catalase-peroxidase HPI from E.
coli was used as an alternative enzyme in polymerization reactions for the production of (−) epicatechin oligomers and their biological activity was characterized.
The enzyme was prepared in two forms: a purified and an immobilized form.
Both were tested for their activity in oxidative polymerization reactions, and their stability and reusability were assessed.
The polymerization reactions were followed by SEC-HPLC analyses, and the substrate was completely converted into one or more polymerization products depending on the reactions conditions.
Results showed that the utilized conditions allowed for the isolation of some oligomers of different molecular weight: the oligomers containing 6 and 7 units of epicatechin substrate are the heaviest ones.
Epicatechin was also used in reactions catalyzed by HRP in the same reaction conditions for comparison.
In addition, one selected oligomer obtained by HPI enzyme catalysis was shown to act as in vitro inhibitor of tumor cell growth, like one oligomer deriving from epicatechin by HRP catalysis.
These data confirm that epicatechin oligomeric form is more effective than its monomer in biological activity and suggest the use of HPI as an alternative enzyme in reactions for the production of epicatechin oligomers.
American Psychological Association (APA)
Di Gennaro, Patrizia& Sabatini, Valentina& Fallarini, Silvia& Pagliarin, Roberto& Sello, Guido. 2018. Polyphenol Polymerization by an Alternative Oxidative Microbial Enzyme and Characterization of the Biological Activity of Oligomers. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126137
Modern Language Association (MLA)
Di Gennaro, Patrizia…[et al.]. Polyphenol Polymerization by an Alternative Oxidative Microbial Enzyme and Characterization of the Biological Activity of Oligomers. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1126137
American Medical Association (AMA)
Di Gennaro, Patrizia& Sabatini, Valentina& Fallarini, Silvia& Pagliarin, Roberto& Sello, Guido. Polyphenol Polymerization by an Alternative Oxidative Microbial Enzyme and Characterization of the Biological Activity of Oligomers. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126137
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126137