Effects and Mechanism of lncRNA CRNDE on Sepsis-Induced Acute Kidney Injury
المؤلفون المشاركون
Wu, Shen
Qiu, Hengyi
Wang, Qiao
Cao, Zheng
Wang, Jianmin
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-01
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Objective.
To investigate the effects of lncRNA CRNDE on sepsis-associated acute kidney injury in the human kidney 2 cell line and explore the potential mechanisms.
Methods.
HK-2 cells were treated with lipopolysaccharides to induce injuries.
The expression of CRNDE and miR-146a in HK-2 cells were altered by a transient transfection assay.
Cell apoptosis was detected by a flow cytometry assay, and the levels of inflammatory cytokines including TNF-α, IL-6, IL-8, and IL-1β were assessed by ELISA.
Furthermore, western blot analysis was performed to detect the expression levels of TLR4/NF-κB pathway-related proteins.
And a luciferase reporter gene assay was used to verify if miR-146a was the target of CRNDE.
Results.
LPS treatment increased CRNDE expression in HK-2 cells.
CRNDE overexpression enhanced cell injuries in HK-2 cells significantly increasing inflammatory cytokine levels, including TNF-α, IL-6, IL-8, and IL-1β, and cell apoptosis.
In addition, CRNDE overexpression further activated the TLR4/NF-κB pathways in HK-2 cells.
Inversely, opposite results were observed in the miR-146a mimic treatment group, and the miR-146a inhibitor could reverse the protein expression changes of TLR4/NF-κB in the si-CRNDE and LPS treatment group.
Conclusion.
This study demonstrated that CRNDE overexpression could activate the TLR4/NF-κB signaling pathway by regulating miR-146a, which accelerated LPS-induced inflammation and apoptosis in HK-2 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Shen& Qiu, Hengyi& Wang, Qiao& Cao, Zheng& Wang, Jianmin. 2020. Effects and Mechanism of lncRNA CRNDE on Sepsis-Induced Acute Kidney Injury. Analytical Cellular Pathology،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1126210
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Shen…[et al.]. Effects and Mechanism of lncRNA CRNDE on Sepsis-Induced Acute Kidney Injury. Analytical Cellular Pathology No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1126210
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Shen& Qiu, Hengyi& Wang, Qiao& Cao, Zheng& Wang, Jianmin. Effects and Mechanism of lncRNA CRNDE on Sepsis-Induced Acute Kidney Injury. Analytical Cellular Pathology. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1126210
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126210
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر