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Effects and Mechanism of lncRNA CRNDE on Sepsis-Induced Acute Kidney Injury
Joint Authors
Wu, Shen
Qiu, Hengyi
Wang, Qiao
Cao, Zheng
Wang, Jianmin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
To investigate the effects of lncRNA CRNDE on sepsis-associated acute kidney injury in the human kidney 2 cell line and explore the potential mechanisms.
Methods.
HK-2 cells were treated with lipopolysaccharides to induce injuries.
The expression of CRNDE and miR-146a in HK-2 cells were altered by a transient transfection assay.
Cell apoptosis was detected by a flow cytometry assay, and the levels of inflammatory cytokines including TNF-α, IL-6, IL-8, and IL-1β were assessed by ELISA.
Furthermore, western blot analysis was performed to detect the expression levels of TLR4/NF-κB pathway-related proteins.
And a luciferase reporter gene assay was used to verify if miR-146a was the target of CRNDE.
Results.
LPS treatment increased CRNDE expression in HK-2 cells.
CRNDE overexpression enhanced cell injuries in HK-2 cells significantly increasing inflammatory cytokine levels, including TNF-α, IL-6, IL-8, and IL-1β, and cell apoptosis.
In addition, CRNDE overexpression further activated the TLR4/NF-κB pathways in HK-2 cells.
Inversely, opposite results were observed in the miR-146a mimic treatment group, and the miR-146a inhibitor could reverse the protein expression changes of TLR4/NF-κB in the si-CRNDE and LPS treatment group.
Conclusion.
This study demonstrated that CRNDE overexpression could activate the TLR4/NF-κB signaling pathway by regulating miR-146a, which accelerated LPS-induced inflammation and apoptosis in HK-2 cells.
American Psychological Association (APA)
Wu, Shen& Qiu, Hengyi& Wang, Qiao& Cao, Zheng& Wang, Jianmin. 2020. Effects and Mechanism of lncRNA CRNDE on Sepsis-Induced Acute Kidney Injury. Analytical Cellular Pathology،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1126210
Modern Language Association (MLA)
Wu, Shen…[et al.]. Effects and Mechanism of lncRNA CRNDE on Sepsis-Induced Acute Kidney Injury. Analytical Cellular Pathology No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1126210
American Medical Association (AMA)
Wu, Shen& Qiu, Hengyi& Wang, Qiao& Cao, Zheng& Wang, Jianmin. Effects and Mechanism of lncRNA CRNDE on Sepsis-Induced Acute Kidney Injury. Analytical Cellular Pathology. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1126210
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126210