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Inhibitory Effects of Genistein on Vascular Smooth Muscle Cell Proliferation Induced by Ox-LDL: Role of BKCa Channels
المؤلفون المشاركون
Bai, Bing
Lu, Nanjuan
Zhang, Wei
Lin, Jinghan
Zhao, Tingting
Zhou, Shanshan
Khasanova, Elona
Zhang, Liming
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-14
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Background.
Oxidized low-density lipoprotein (Ox-LDL) is a crucial pathogenic factor for vascular diseases, which can induce the proliferation of vascular smooth muscle cells (VSMCs).
Genistein is the main component of soybean isoflavone.
Genistein has a variety of pharmacological properties in the treatment of vascular diseases and a promising clinical application.
Large-conductance calcium-activated potassium (BKCa) channels are the primary type of potassium channels in VSMCs, which regulate various biological functions of VSMCs.
However, whether genistein exerts an antiproliferation effect on Ox-LDL-stimulated VSMCs remains unclear.
The current study is aimed at elucidating the effect of genistein on the Ox-LDL-stimulated proliferation of VSMCs and its possible molecular mechanism, especially the electrophysiological mechanism related to BKCa channels.
Methods.
Monoculture VSMC was obtained by an acute enzyme-dispersing method.
The proliferation of cells was measured by CCK-8, cell cycle, and proliferating cell nuclear antigen (PCNA) expression.
The BKCa whole-cell currents were measured by patch-clamp.
Results.
Ox-LDL treatment induced the proliferation of VSMCs, upregulated the BKCa protein expression, and increased the density of BKCa currents, while genistein significantly inhibited these effects caused by Ox-LDL.
BKCa channels exerted a regulatory role in the proliferation of VSMCs in response to Ox-LDL.
The inhibition of BKCa channels suppressed Ox-LDL-stimulated VSMC proliferation, while the activation of BKCa channels showed the opposite effect.
Moreover, genistein suppressed the activity of BKCa, including protein expression and current density in a protein tyrosine kinase- (PTK-) dependent manner.
Conclusion.
This study demonstrated that genistein inhibited the Ox-LDL-mediated proliferation of VSMCs by blocking the cell cycle progression; the possible molecular mechanism may be related to PTK-dependent suppression of BKCa channels.
Our results provided novel ideas for the application of genistein in the treatment of vascular diseases and proposed a unique insight into the antiproliferative molecular mechanism of genistein.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bai, Bing& Lu, Nanjuan& Zhang, Wei& Lin, Jinghan& Zhao, Tingting& Zhou, Shanshan…[et al.]. 2020. Inhibitory Effects of Genistein on Vascular Smooth Muscle Cell Proliferation Induced by Ox-LDL: Role of BKCa Channels. Analytical Cellular Pathology،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1126219
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bai, Bing…[et al.]. Inhibitory Effects of Genistein on Vascular Smooth Muscle Cell Proliferation Induced by Ox-LDL: Role of BKCa Channels. Analytical Cellular Pathology No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1126219
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bai, Bing& Lu, Nanjuan& Zhang, Wei& Lin, Jinghan& Zhao, Tingting& Zhou, Shanshan…[et al.]. Inhibitory Effects of Genistein on Vascular Smooth Muscle Cell Proliferation Induced by Ox-LDL: Role of BKCa Channels. Analytical Cellular Pathology. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1126219
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126219
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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