Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish
المؤلفون المشاركون
Yin, Heng
Wang, Jianwei
Wu, Mao
Ma, Yong
Wang, Shanfu
Su, Qiuju
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-22
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
The aim of this study was to investigate the effect of evodiamine (EV) on dexamethasone-induced osteoporosis in zebrafish.
Zebrafish larvae were exposed to different concentrations of dexamethasone to obtain the osteoporosis in zebrafish.
Calcium, phosphorus, and alizarin red staining determination were performed to evaluate the effects of EV on bone mineralization.
Alkaline phosphatase (ALP), hydroxyproline (HP), and tartrate resistant acid phosphatase (TRAP) were also measured by commercial kits.
The expression of MMP3-OPN-MAPK pathway in zebrafish was measured by Western blot.
RT-PCR was used to determine mRNA levels of MMP3, OPN, and MAPK.
EV could significantly increase the content of calcium and phosphorus.
The results of alizarin red staining showed that EV could significantly increase the calcium sink of horse fish, increasing the area of bone formation.
EV could increase the content of hydroxyproline in zebrafish.
EV also increased ALP and TRAP in zebrafish.
Western blot and RT-PCR results showed that EV restored the MMP3-OPN-MAPK pathway in zebrafish.
In conclusion, we found that EV can alleviate dexamethasone-induced osteoporosis in zebrafish.
The mechanism is related to activating MMP3-OPN-MAPK pathway and then activating bone remodeling.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yin, Heng& Wang, Jianwei& Wu, Mao& Ma, Yong& Wang, Shanfu& Su, Qiuju. 2019. Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish. BioMed Research International،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1126289
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yin, Heng…[et al.]. Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish. BioMed Research International No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1126289
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yin, Heng& Wang, Jianwei& Wu, Mao& Ma, Yong& Wang, Shanfu& Su, Qiuju. Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1126289
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126289
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر