Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish
Joint Authors
Yin, Heng
Wang, Jianwei
Wu, Mao
Ma, Yong
Wang, Shanfu
Su, Qiuju
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-22
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The aim of this study was to investigate the effect of evodiamine (EV) on dexamethasone-induced osteoporosis in zebrafish.
Zebrafish larvae were exposed to different concentrations of dexamethasone to obtain the osteoporosis in zebrafish.
Calcium, phosphorus, and alizarin red staining determination were performed to evaluate the effects of EV on bone mineralization.
Alkaline phosphatase (ALP), hydroxyproline (HP), and tartrate resistant acid phosphatase (TRAP) were also measured by commercial kits.
The expression of MMP3-OPN-MAPK pathway in zebrafish was measured by Western blot.
RT-PCR was used to determine mRNA levels of MMP3, OPN, and MAPK.
EV could significantly increase the content of calcium and phosphorus.
The results of alizarin red staining showed that EV could significantly increase the calcium sink of horse fish, increasing the area of bone formation.
EV could increase the content of hydroxyproline in zebrafish.
EV also increased ALP and TRAP in zebrafish.
Western blot and RT-PCR results showed that EV restored the MMP3-OPN-MAPK pathway in zebrafish.
In conclusion, we found that EV can alleviate dexamethasone-induced osteoporosis in zebrafish.
The mechanism is related to activating MMP3-OPN-MAPK pathway and then activating bone remodeling.
American Psychological Association (APA)
Yin, Heng& Wang, Jianwei& Wu, Mao& Ma, Yong& Wang, Shanfu& Su, Qiuju. 2019. Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish. BioMed Research International،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1126289
Modern Language Association (MLA)
Yin, Heng…[et al.]. Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish. BioMed Research International No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1126289
American Medical Association (AMA)
Yin, Heng& Wang, Jianwei& Wu, Mao& Ma, Yong& Wang, Shanfu& Su, Qiuju. Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1126289
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126289