Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish

Joint Authors

Yin, Heng
Wang, Jianwei
Wu, Mao
Ma, Yong
Wang, Shanfu
Su, Qiuju

Source

BioMed Research International

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-01-22

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Medicine

Abstract EN

The aim of this study was to investigate the effect of evodiamine (EV) on dexamethasone-induced osteoporosis in zebrafish.

Zebrafish larvae were exposed to different concentrations of dexamethasone to obtain the osteoporosis in zebrafish.

Calcium, phosphorus, and alizarin red staining determination were performed to evaluate the effects of EV on bone mineralization.

Alkaline phosphatase (ALP), hydroxyproline (HP), and tartrate resistant acid phosphatase (TRAP) were also measured by commercial kits.

The expression of MMP3-OPN-MAPK pathway in zebrafish was measured by Western blot.

RT-PCR was used to determine mRNA levels of MMP3, OPN, and MAPK.

EV could significantly increase the content of calcium and phosphorus.

The results of alizarin red staining showed that EV could significantly increase the calcium sink of horse fish, increasing the area of bone formation.

EV could increase the content of hydroxyproline in zebrafish.

EV also increased ALP and TRAP in zebrafish.

Western blot and RT-PCR results showed that EV restored the MMP3-OPN-MAPK pathway in zebrafish.

In conclusion, we found that EV can alleviate dexamethasone-induced osteoporosis in zebrafish.

The mechanism is related to activating MMP3-OPN-MAPK pathway and then activating bone remodeling.

American Psychological Association (APA)

Yin, Heng& Wang, Jianwei& Wu, Mao& Ma, Yong& Wang, Shanfu& Su, Qiuju. 2019. Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish. BioMed Research International،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1126289

Modern Language Association (MLA)

Yin, Heng…[et al.]. Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish. BioMed Research International No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1126289

American Medical Association (AMA)

Yin, Heng& Wang, Jianwei& Wu, Mao& Ma, Yong& Wang, Shanfu& Su, Qiuju. Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1126289

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1126289