The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy

المؤلفون المشاركون

Qiu, Long-Xin
Guo, Chang
Ma, Jing Fan
Wei, Peng Fei
Shi, Yuan Peng
Lv, Yang
Wen, Long Ping

المصدر

BioMed Research International

العدد

المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2018-05-24

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

Gynura formosana Kitam.

belongs to the Compositae family and has been traditionally used for the prevention of cancer, diabetes, and inflammation in China.

Previous studies had indicated that the ethyl acetate extract of Gynura formosana Kitam.

leaves (EAEG) exhibited antioxidant and anti-inflammatory activity.

In this report, we demonstrated that EAEG possessed potent anticancer activity through autophagy-mediated inhibition of cell proliferation.

EAEG induced a strong cytostatic effect towards HeLa cells and, to a lesser extent, HepG2 and MCF-7 cells.

This cytostatic effect of EAEG was not a consequence of increased apoptosis, as neither DNA fragmentation nor change in protein expression level for a number of apoptosis-related genes including Bid, Bax, Bcl-2, and caspase-3 was observed after EAEG treatment, and the apoptosis inhibitor Z-VAD-FMK did not inhibit the EAEG-elicited cytostatic effect.

On the other hand, EAEG induced autophagy in a dose-dependent fashion, as shown by increased GFP puncta formation, enhanced conversion of the microtubule-associated protein light chain LC3-I to LC3-II, and downregulation of the p62 protein.

Treating the HeLa cells with EAEG together with Chloroquine (CQ) further accelerated LC3 conversion and p62 clearance, indicating that EAEG induced complete autophagy flux.

Importantly, the autophagy inhibitor 3-methyladenine (3MA) significantly abrogated the cytostatic effect of EAEG, strongly suggesting that EAEG inhibited HeLa cell proliferation through the induction of autophagy rather than apoptosis.

Our results provided a novel and interesting mechanistic insight into the anticancer action of EAEG, supporting the traditional use of this plant for the treatment of the cancer.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Ma, Jing Fan& Wei, Peng Fei& Guo, Chang& Shi, Yuan Peng& Lv, Yang& Qiu, Long-Xin…[et al.]. 2018. The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126897

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Ma, Jing Fan…[et al.]. The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1126897

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Ma, Jing Fan& Wei, Peng Fei& Guo, Chang& Shi, Yuan Peng& Lv, Yang& Qiu, Long-Xin…[et al.]. The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126897

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1126897