The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy
Joint Authors
Qiu, Long-Xin
Guo, Chang
Ma, Jing Fan
Wei, Peng Fei
Shi, Yuan Peng
Lv, Yang
Wen, Long Ping
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Gynura formosana Kitam.
belongs to the Compositae family and has been traditionally used for the prevention of cancer, diabetes, and inflammation in China.
Previous studies had indicated that the ethyl acetate extract of Gynura formosana Kitam.
leaves (EAEG) exhibited antioxidant and anti-inflammatory activity.
In this report, we demonstrated that EAEG possessed potent anticancer activity through autophagy-mediated inhibition of cell proliferation.
EAEG induced a strong cytostatic effect towards HeLa cells and, to a lesser extent, HepG2 and MCF-7 cells.
This cytostatic effect of EAEG was not a consequence of increased apoptosis, as neither DNA fragmentation nor change in protein expression level for a number of apoptosis-related genes including Bid, Bax, Bcl-2, and caspase-3 was observed after EAEG treatment, and the apoptosis inhibitor Z-VAD-FMK did not inhibit the EAEG-elicited cytostatic effect.
On the other hand, EAEG induced autophagy in a dose-dependent fashion, as shown by increased GFP puncta formation, enhanced conversion of the microtubule-associated protein light chain LC3-I to LC3-II, and downregulation of the p62 protein.
Treating the HeLa cells with EAEG together with Chloroquine (CQ) further accelerated LC3 conversion and p62 clearance, indicating that EAEG induced complete autophagy flux.
Importantly, the autophagy inhibitor 3-methyladenine (3MA) significantly abrogated the cytostatic effect of EAEG, strongly suggesting that EAEG inhibited HeLa cell proliferation through the induction of autophagy rather than apoptosis.
Our results provided a novel and interesting mechanistic insight into the anticancer action of EAEG, supporting the traditional use of this plant for the treatment of the cancer.
American Psychological Association (APA)
Ma, Jing Fan& Wei, Peng Fei& Guo, Chang& Shi, Yuan Peng& Lv, Yang& Qiu, Long-Xin…[et al.]. 2018. The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126897
Modern Language Association (MLA)
Ma, Jing Fan…[et al.]. The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1126897
American Medical Association (AMA)
Ma, Jing Fan& Wei, Peng Fei& Guo, Chang& Shi, Yuan Peng& Lv, Yang& Qiu, Long-Xin…[et al.]. The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126897
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126897