Potassium Channel Activation Is Involved in the Cardiovascular Effects Induced by Freeze Dried Syzygium jambolanum (Lam.)‎ DC Fruit Juice

الملخص EN

This work aimed to explore the cardiovascular effects induced by freeze-dried juice from Syzygium jambolanum (Lam.) DC fruits (JSJ).

JSJ presented high polyphenol content and steroids.

HPLC analysis revealed that 2,5-dihydroxybenzoic and caffeic acid were present in higher amounts in the JSJ extract.

In rat, JSJ induces hypotension and vasodilatation in mesenteric arteries, with or without vascular endothelium.

JSJ-mediated vasodilation response against contractions induced with KCl (60 mM) depolarizing solution was significantly lower than the responses induced by JSJ when evaluated against phenylephrine-induced contractions.

To investigate the involvement of potassium channels we used Tyrode’s solution with KCl (20 mM) or tetraethylammonium (1.0, 3.0, or 5.0 mM).

In these conditions JSJ-induced effects were significantly attenuated.

To investigate the potassium channel subtypes involved in the response, we used 4-aminopyridine, glibenclamide, BaCl2, and iberiotoxin.

In the presence (simultaneous) of different potassium channel blockers we observed a significant attenuation of JSJ-induced effect.

Inhibition was also observed when using BaCl2, glibenclamide, or 4-aminopyridine, separately.

However, incubation with iberiotoxin did not promote changes in either maximum effect, or potency.

We also evidenced a discrete participation of CaV channels in the JSJ-induced vasorelaxant effect.

In addition, patch-clamp studies demonstrated that JSJ could activate potassium channels.

In conclusion, JSJ promotes hypotension and vasorelaxation in rats, involving, at least, the activation of potassium channels.