Increased Autophagy Enhances the Resistance to Tumor Necrosis Factor-Alpha Treatment in Rheumatoid Arthritis Human Fibroblast-Like Synovial Cell
المؤلفون المشاركون
Hu, Shaoxian
Dai, Yujie
Ding, Jingjing
Yin, Wen
He, Yuzhu
Yu, Fei
Ye, Cong
Yu, Yikai
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-10-25
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Tumor Necrosis Factor-alpha (TNF-α) was reported to increase autophagy in rheumatoid arthritis human fibroblast-like synovial cell (RA-HFLS).
We investigated different levels of TNF-α exposed to RA-HFLS by focusing on the relationship of autophagy and apoptosis.
RA-HFLS and normal human fibroblast-like synovial cell (HFLS) were stimulated by TNF-α in the presence or the absence of 3-methyladenine (3-MA) or chloroquine (CQ).
Cell apoptosis was detected by flow cytometry.
Autophagy was determined through the expression levels of LC3, Beclin1, and P62 measured by Western Blot analysis as well as Confocal Laser Scanning Microscopy.
The basal autophagy level was significantly higher in RA-HFLS than in HFLS.
Autophagy was enhanced both in RA-HFLS and HFLS when they were treated with TNF-α.
With the treatment of TNF-α, a slightly higher autophagy level was found in RA-HFLS than in HFLS, without a dose dependent effect.
When autophagy was inhibited by 3-MA or CQ, apoptosis increased in both groups.
With the stimulation of different doses TNF-α, apoptosis was much higher in HFLS group than in RA-HFLS.
Autophagy is a protection mechanism when treated by TNF-α in RA-HFLS.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dai, Yujie& Ding, Jingjing& Yin, Wen& He, Yuzhu& Yu, Fei& Ye, Cong…[et al.]. 2018. Increased Autophagy Enhances the Resistance to Tumor Necrosis Factor-Alpha Treatment in Rheumatoid Arthritis Human Fibroblast-Like Synovial Cell. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127005
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dai, Yujie…[et al.]. Increased Autophagy Enhances the Resistance to Tumor Necrosis Factor-Alpha Treatment in Rheumatoid Arthritis Human Fibroblast-Like Synovial Cell. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1127005
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dai, Yujie& Ding, Jingjing& Yin, Wen& He, Yuzhu& Yu, Fei& Ye, Cong…[et al.]. Increased Autophagy Enhances the Resistance to Tumor Necrosis Factor-Alpha Treatment in Rheumatoid Arthritis Human Fibroblast-Like Synovial Cell. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127005
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1127005
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر