Increased Autophagy Enhances the Resistance to Tumor Necrosis Factor-Alpha Treatment in Rheumatoid Arthritis Human Fibroblast-Like Synovial Cell
Joint Authors
Hu, Shaoxian
Dai, Yujie
Ding, Jingjing
Yin, Wen
He, Yuzhu
Yu, Fei
Ye, Cong
Yu, Yikai
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Tumor Necrosis Factor-alpha (TNF-α) was reported to increase autophagy in rheumatoid arthritis human fibroblast-like synovial cell (RA-HFLS).
We investigated different levels of TNF-α exposed to RA-HFLS by focusing on the relationship of autophagy and apoptosis.
RA-HFLS and normal human fibroblast-like synovial cell (HFLS) were stimulated by TNF-α in the presence or the absence of 3-methyladenine (3-MA) or chloroquine (CQ).
Cell apoptosis was detected by flow cytometry.
Autophagy was determined through the expression levels of LC3, Beclin1, and P62 measured by Western Blot analysis as well as Confocal Laser Scanning Microscopy.
The basal autophagy level was significantly higher in RA-HFLS than in HFLS.
Autophagy was enhanced both in RA-HFLS and HFLS when they were treated with TNF-α.
With the treatment of TNF-α, a slightly higher autophagy level was found in RA-HFLS than in HFLS, without a dose dependent effect.
When autophagy was inhibited by 3-MA or CQ, apoptosis increased in both groups.
With the stimulation of different doses TNF-α, apoptosis was much higher in HFLS group than in RA-HFLS.
Autophagy is a protection mechanism when treated by TNF-α in RA-HFLS.
American Psychological Association (APA)
Dai, Yujie& Ding, Jingjing& Yin, Wen& He, Yuzhu& Yu, Fei& Ye, Cong…[et al.]. 2018. Increased Autophagy Enhances the Resistance to Tumor Necrosis Factor-Alpha Treatment in Rheumatoid Arthritis Human Fibroblast-Like Synovial Cell. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127005
Modern Language Association (MLA)
Dai, Yujie…[et al.]. Increased Autophagy Enhances the Resistance to Tumor Necrosis Factor-Alpha Treatment in Rheumatoid Arthritis Human Fibroblast-Like Synovial Cell. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1127005
American Medical Association (AMA)
Dai, Yujie& Ding, Jingjing& Yin, Wen& He, Yuzhu& Yu, Fei& Ye, Cong…[et al.]. Increased Autophagy Enhances the Resistance to Tumor Necrosis Factor-Alpha Treatment in Rheumatoid Arthritis Human Fibroblast-Like Synovial Cell. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127005
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127005