PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF- κ B Signaling Pathway
المؤلفون المشاركون
Duan, Yang
Tan, Zhiwen
Yang, Minsheng
Li, Jianjun
Liu, Chun
Wang, Chengqiang
Zhang, Fu
Jin, Yanglei
Wang, Yihan
Zhu, Lixin
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-01
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Prostate cancer is a serious disease that can invade bone tissues.
These bone metastases can greatly decrease a patient’s quality of life, pose a financial burden, and even result in death.
In recent years, tumor cell-secreted microvesicles have been identified and proposed to be a key factor in cell interaction.
However, the impact of cancer-derived exosomes on bone cells remains unclear.
Herein, we isolated exosomes from prostate cancer cell line PC-3 and investigated their effects on human osteoclast differentiation by tartrate-resistant acid phosphatase (TRAP) staining.
The potential mechanism was evaluated by qRT-PCR, western blotting, and microRNA transfection experiments.
The results showed that PC-3-derived exosomes dramatically inhibited osteoclast differentiation.
Marker genes of mature osteoclasts, including CTSK, NFATc1, ACP5, and miR-214, were all downregulated in the presence of PC-3 exosomes.
Furthermore, transfection experiments showed that miR-214 downregulation severely impaired osteoclast differentiation, whereas overexpression of miR-214 promoted differentiation.
Furthermore, we demonstrated that PC-3-derived exosomes block the NF-κB signaling pathway.
Our study suggested that PC-3-derived exosomes inhibit osteoclast differentiation by downregulating miR-214 and blocking the NF-κB signaling pathway.
Therefore, elevating miR-214 levels in the bone metastatic site may attenuate the invasion of prostate cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Duan, Yang& Tan, Zhiwen& Yang, Minsheng& Li, Jianjun& Liu, Chun& Wang, Chengqiang…[et al.]. 2019. PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF- κ B Signaling Pathway. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128056
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Duan, Yang…[et al.]. PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF- κ B Signaling Pathway. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1128056
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Duan, Yang& Tan, Zhiwen& Yang, Minsheng& Li, Jianjun& Liu, Chun& Wang, Chengqiang…[et al.]. PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF- κ B Signaling Pathway. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128056
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128056
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر