PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF- κ B Signaling Pathway
Joint Authors
Duan, Yang
Tan, Zhiwen
Yang, Minsheng
Li, Jianjun
Liu, Chun
Wang, Chengqiang
Zhang, Fu
Jin, Yanglei
Wang, Yihan
Zhu, Lixin
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Prostate cancer is a serious disease that can invade bone tissues.
These bone metastases can greatly decrease a patient’s quality of life, pose a financial burden, and even result in death.
In recent years, tumor cell-secreted microvesicles have been identified and proposed to be a key factor in cell interaction.
However, the impact of cancer-derived exosomes on bone cells remains unclear.
Herein, we isolated exosomes from prostate cancer cell line PC-3 and investigated their effects on human osteoclast differentiation by tartrate-resistant acid phosphatase (TRAP) staining.
The potential mechanism was evaluated by qRT-PCR, western blotting, and microRNA transfection experiments.
The results showed that PC-3-derived exosomes dramatically inhibited osteoclast differentiation.
Marker genes of mature osteoclasts, including CTSK, NFATc1, ACP5, and miR-214, were all downregulated in the presence of PC-3 exosomes.
Furthermore, transfection experiments showed that miR-214 downregulation severely impaired osteoclast differentiation, whereas overexpression of miR-214 promoted differentiation.
Furthermore, we demonstrated that PC-3-derived exosomes block the NF-κB signaling pathway.
Our study suggested that PC-3-derived exosomes inhibit osteoclast differentiation by downregulating miR-214 and blocking the NF-κB signaling pathway.
Therefore, elevating miR-214 levels in the bone metastatic site may attenuate the invasion of prostate cancer.
American Psychological Association (APA)
Duan, Yang& Tan, Zhiwen& Yang, Minsheng& Li, Jianjun& Liu, Chun& Wang, Chengqiang…[et al.]. 2019. PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF- κ B Signaling Pathway. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128056
Modern Language Association (MLA)
Duan, Yang…[et al.]. PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF- κ B Signaling Pathway. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1128056
American Medical Association (AMA)
Duan, Yang& Tan, Zhiwen& Yang, Minsheng& Li, Jianjun& Liu, Chun& Wang, Chengqiang…[et al.]. PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF- κ B Signaling Pathway. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128056
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128056