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Upregulated TSG-6 Expression in ADSCs Inhibits the BV2 Microglia-Mediated Inflammatory Response
المؤلفون المشاركون
Hu, Yang
Pan, Chao
Tang, Zhouping
Li, Gaigai
Zhang, Ye
Liu, Na
Zhang, Ping
Nie, Hao
Li, Qi
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-11-21
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Objectives.
The microglial cells are immune surveillance cells in the central nervous system and can be activated during neurological disorders.
Adipose-derived stem cells (ADSCs) were reported to inhibit the inflammatory response in microglia by secreting proteins like tumor necrosis factor-inducible gene 6 protein (TSG-6).
We aim to explore the mechanisms and the associated microRNAs.
Methods.
ADSCs were cultured and TSG-6 expression was evaluated.
ADSCs were cocultured with lipopolysaccharide- (LPS-) induced BV2 microglia and the supernatant was harvested for detecting cytokines.
The total RNA was extracted and sequenced by high-throughput sequencing.
MicroRNA profiles were compared between two treatment groups of ADSCs.
A comprehensive bioinformatics analysis and confirmation experiments were performed to identify the microRNAs targeting at TSG-6.
Results.
We found that ADSCs could secrete TSG-6 to inhibit the proinflammatory cytokines, including interleukin-1 beta and interleukin-6, and tumor necrosis factor alpha (TNFα), produced by LPS-induced microglia-mediated inflammatory response.
Bioinformatics analysis showed a total of 35 microRNAs differentially expressed between the two groups of ADSCs, and miR-214-5p was identified as a regulator of TSG-6 mRNA.
Conclusion.
Following a treatment with TNFα, ADSCs can regulate the inflammatory response in LPS-activated BV2 microglia by upregulating TSG-6 expression, which itself is under the negative control of miR-214-5p.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hu, Yang& Li, Gaigai& Zhang, Ye& Liu, Na& Zhang, Ping& Pan, Chao…[et al.]. 2018. Upregulated TSG-6 Expression in ADSCs Inhibits the BV2 Microglia-Mediated Inflammatory Response. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1128346
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hu, Yang…[et al.]. Upregulated TSG-6 Expression in ADSCs Inhibits the BV2 Microglia-Mediated Inflammatory Response. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1128346
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hu, Yang& Li, Gaigai& Zhang, Ye& Liu, Na& Zhang, Ping& Pan, Chao…[et al.]. Upregulated TSG-6 Expression in ADSCs Inhibits the BV2 Microglia-Mediated Inflammatory Response. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1128346
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128346
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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