Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of Burkholderia pseudomallei and Leptospira spp. DNA
المؤلفون المشاركون
Yean Yean, Chan
Goay, Yuan Xin
Phua, Kia Kien
Foo, Phiaw Chong
Harun, Azian
Ismail, Nabilah
Mohd Ali, Mohammad Ridhuan
Lih Huey, Lee
Ismail, Asmaliza S.
Mustaffa, Khairul Mohd Fadzli
Aziah, Ismail
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-07-02
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Melioidosis and leptospirosis, caused by two different bacteria, Burkholderia pseudomallei and Leptospira spp., are potentially fatal infections that share a very similar spectrum of clinical features and cause significant mortality and morbidity in humans and livestock.
Early detection is important for better clinical consequences.
To our knowledge, there is no diagnostic tool available to simultaneously detect and differentiate melioidosis and leptospirosis in humans and animals.
In this study, we described a duplex TaqMan probe-based qPCR for the detection of B.
pseudomallei and Leptospira spp.
DNA.
The performance of the assay was evaluated on 20 B.
pseudomallei isolates, 23 Leptospira strains, and 39 other microorganisms, as well as two sets of serially diluted reference strains.
The duplex qPCR assay was able to detect 0.02 pg (~ 4 copies) Leptospira spp.
DNA and 0.2 pg (~ 25.6 copies) B.
pseudomallei DNA.
No undesired amplification was observed in other microorganisms.
In conclusion, the duplex qPCR assay was sensitive and specific for the detection of B.
pseudomallei & Leptospira spp.
DNA and is suitable for further analytical and clinical evaluation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mohd Ali, Mohammad Ridhuan& Lih Huey, Lee& Foo, Phiaw Chong& Goay, Yuan Xin& Ismail, Asmaliza S.& Mustaffa, Khairul Mohd Fadzli…[et al.]. 2019. Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of Burkholderia pseudomallei and Leptospira spp. DNA. BioMed Research International،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1128553
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mohd Ali, Mohammad Ridhuan…[et al.]. Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of Burkholderia pseudomallei and Leptospira spp. DNA. BioMed Research International No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1128553
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mohd Ali, Mohammad Ridhuan& Lih Huey, Lee& Foo, Phiaw Chong& Goay, Yuan Xin& Ismail, Asmaliza S.& Mustaffa, Khairul Mohd Fadzli…[et al.]. Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of Burkholderia pseudomallei and Leptospira spp. DNA. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1128553
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128553
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر