Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of Burkholderia pseudomallei and Leptospira spp. DNA
Joint Authors
Yean Yean, Chan
Goay, Yuan Xin
Phua, Kia Kien
Foo, Phiaw Chong
Harun, Azian
Ismail, Nabilah
Mohd Ali, Mohammad Ridhuan
Lih Huey, Lee
Ismail, Asmaliza S.
Mustaffa, Khairul Mohd Fadzli
Aziah, Ismail
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-02
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Melioidosis and leptospirosis, caused by two different bacteria, Burkholderia pseudomallei and Leptospira spp., are potentially fatal infections that share a very similar spectrum of clinical features and cause significant mortality and morbidity in humans and livestock.
Early detection is important for better clinical consequences.
To our knowledge, there is no diagnostic tool available to simultaneously detect and differentiate melioidosis and leptospirosis in humans and animals.
In this study, we described a duplex TaqMan probe-based qPCR for the detection of B.
pseudomallei and Leptospira spp.
DNA.
The performance of the assay was evaluated on 20 B.
pseudomallei isolates, 23 Leptospira strains, and 39 other microorganisms, as well as two sets of serially diluted reference strains.
The duplex qPCR assay was able to detect 0.02 pg (~ 4 copies) Leptospira spp.
DNA and 0.2 pg (~ 25.6 copies) B.
pseudomallei DNA.
No undesired amplification was observed in other microorganisms.
In conclusion, the duplex qPCR assay was sensitive and specific for the detection of B.
pseudomallei & Leptospira spp.
DNA and is suitable for further analytical and clinical evaluation.
American Psychological Association (APA)
Mohd Ali, Mohammad Ridhuan& Lih Huey, Lee& Foo, Phiaw Chong& Goay, Yuan Xin& Ismail, Asmaliza S.& Mustaffa, Khairul Mohd Fadzli…[et al.]. 2019. Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of Burkholderia pseudomallei and Leptospira spp. DNA. BioMed Research International،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1128553
Modern Language Association (MLA)
Mohd Ali, Mohammad Ridhuan…[et al.]. Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of Burkholderia pseudomallei and Leptospira spp. DNA. BioMed Research International No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1128553
American Medical Association (AMA)
Mohd Ali, Mohammad Ridhuan& Lih Huey, Lee& Foo, Phiaw Chong& Goay, Yuan Xin& Ismail, Asmaliza S.& Mustaffa, Khairul Mohd Fadzli…[et al.]. Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of Burkholderia pseudomallei and Leptospira spp. DNA. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1128553
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128553