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Follistatin-Like 1 Attenuates IschemiaReperfusion Injury in Cardiomyocytes via Regulation of Autophagy
المؤلفون المشاركون
Yang, Weijun
Duan, Qunjun
Zhu, Xian
Tao, Kaiyu
Dong, Aiqiang
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-21
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background.
The cardioprotective effect of FSTL1 has been extensively studied in recent years, but its role in myocardial ischemia/reperfusion injury (IRI) is unclear.
In this study, we investigated the effect of FSTL1 pretreatment on myocardial IRI as well as the possible involvement of autophagic pathways in its effects.
Methods.
The effects of FSTL1 on the viability and apoptosis of rat cardiomyocytes were investigated after exposure of cardiomyocytes to hypoxia/ischemia by using the CCK-8 assay and Annexin V/PI staining.
Further, western blot analysis was used to detect the effects of FSTL1 pretreatment on autophagy-associated proteins, and confocal microscopy was used to observe autophagic flux.
To confirm the role of autophagy, the cells were treated with the autophagy promoter rapamycin or the autophagy inhibitor 3-methyladenine, and cell viability and apoptosis during IRI were observed.
These effects were also observed after treatment with rapamycin or 3-methyladenine followed by FSTL1 administration and IRI.
Results.
FSTL1 pretreatment significantly increased viability and reduced apoptosis in cardiomyocytes exposed to hypoxia/ischemia conditions.
Further, FSTL1 pretreatment affected the levels of the autophagy-related proteins and enhanced autophagic flux during IRI.
In addition, cell viability was enhanced and apoptosis was decreased by rapamycin treatment, while these effects were reversed by 3-MA treatment.
However, when the myocardial cells were pretreated with rapamycin or 3-methyladenine, there was no significant change in their viability or apoptosis with FSTL1 treatment during IRI.
Conclusions.
FSTL1 plays a protective role in myocardial IRI by regulating autophagy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Weijun& Duan, Qunjun& Zhu, Xian& Tao, Kaiyu& Dong, Aiqiang. 2019. Follistatin-Like 1 Attenuates IschemiaReperfusion Injury in Cardiomyocytes via Regulation of Autophagy. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128621
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Weijun…[et al.]. Follistatin-Like 1 Attenuates IschemiaReperfusion Injury in Cardiomyocytes via Regulation of Autophagy. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1128621
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Weijun& Duan, Qunjun& Zhu, Xian& Tao, Kaiyu& Dong, Aiqiang. Follistatin-Like 1 Attenuates IschemiaReperfusion Injury in Cardiomyocytes via Regulation of Autophagy. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128621
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128621
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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