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Follistatin-Like 1 Attenuates IschemiaReperfusion Injury in Cardiomyocytes via Regulation of Autophagy
Joint Authors
Yang, Weijun
Duan, Qunjun
Zhu, Xian
Tao, Kaiyu
Dong, Aiqiang
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
The cardioprotective effect of FSTL1 has been extensively studied in recent years, but its role in myocardial ischemia/reperfusion injury (IRI) is unclear.
In this study, we investigated the effect of FSTL1 pretreatment on myocardial IRI as well as the possible involvement of autophagic pathways in its effects.
Methods.
The effects of FSTL1 on the viability and apoptosis of rat cardiomyocytes were investigated after exposure of cardiomyocytes to hypoxia/ischemia by using the CCK-8 assay and Annexin V/PI staining.
Further, western blot analysis was used to detect the effects of FSTL1 pretreatment on autophagy-associated proteins, and confocal microscopy was used to observe autophagic flux.
To confirm the role of autophagy, the cells were treated with the autophagy promoter rapamycin or the autophagy inhibitor 3-methyladenine, and cell viability and apoptosis during IRI were observed.
These effects were also observed after treatment with rapamycin or 3-methyladenine followed by FSTL1 administration and IRI.
Results.
FSTL1 pretreatment significantly increased viability and reduced apoptosis in cardiomyocytes exposed to hypoxia/ischemia conditions.
Further, FSTL1 pretreatment affected the levels of the autophagy-related proteins and enhanced autophagic flux during IRI.
In addition, cell viability was enhanced and apoptosis was decreased by rapamycin treatment, while these effects were reversed by 3-MA treatment.
However, when the myocardial cells were pretreated with rapamycin or 3-methyladenine, there was no significant change in their viability or apoptosis with FSTL1 treatment during IRI.
Conclusions.
FSTL1 plays a protective role in myocardial IRI by regulating autophagy.
American Psychological Association (APA)
Yang, Weijun& Duan, Qunjun& Zhu, Xian& Tao, Kaiyu& Dong, Aiqiang. 2019. Follistatin-Like 1 Attenuates IschemiaReperfusion Injury in Cardiomyocytes via Regulation of Autophagy. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128621
Modern Language Association (MLA)
Yang, Weijun…[et al.]. Follistatin-Like 1 Attenuates IschemiaReperfusion Injury in Cardiomyocytes via Regulation of Autophagy. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1128621
American Medical Association (AMA)
Yang, Weijun& Duan, Qunjun& Zhu, Xian& Tao, Kaiyu& Dong, Aiqiang. Follistatin-Like 1 Attenuates IschemiaReperfusion Injury in Cardiomyocytes via Regulation of Autophagy. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128621
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128621