Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells
المؤلفون المشاركون
Liu, Lin
Liu, Kun
Yan, Yanzhe
Chu, Zhuangzhuang
Tang, Yi
Tang, Chunbo
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-19
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Objectives.
Enhanced migration and osteogenic differentiation of mesenchymal stem cells (MSCs) are beneficial for MSC-mediated periodontal tissue regeneration, a promising method for periodontitis treatment.
FBXO5, a member of the F-box protein family, is involved in the osteogenic differentiation of MSCs.
Here, we investigated the effect of FBXO5 on human periodontal ligament stem cells (hPDLSCs).
Materials and Methods.
hPDLSCs were isolated from periodontal ligament tissue.
Lentivirus FBXO5 shRNA was used to silence FBXO5 expression.
Two transcripts of FBXO5 were overexpressed and transduced into hPDLSCs via retroviral infection.
Migration and osteogenic differentiation of hPDLSCs were evaluated using the scratch migration assay, alkaline phosphatase (ALP) activity, ALP staining, alizarin red staining, western blotting, and real-time polymerase chain reaction.
Results.
The expression of FBXO5 was upregulated after osteogenic induction in hPDLSCs.
FBXO5 knockdown attenuated migration, inhibited ALP activity and mineralization, and decreased RUNX2, OSX, and OCN expression, while the overexpression of two transcript isoforms significantly accelerated migration, enhanced ALP activity and mineralization, and increased RUNX2, OSX, and OCN expression in hPDLSCs.
Conclusions.
Both isoforms of FBXO5 promoted the migration and osteogenic differentiation potential of hPDLSCs, which identified a potential target for improving periodontal tissue regeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Lin& Liu, Kun& Yan, Yanzhe& Chu, Zhuangzhuang& Tang, Yi& Tang, Chunbo. 2018. Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1128740
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Lin…[et al.]. Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1128740
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Lin& Liu, Kun& Yan, Yanzhe& Chu, Zhuangzhuang& Tang, Yi& Tang, Chunbo. Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1128740
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128740
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر