Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells
Joint Authors
Liu, Lin
Liu, Kun
Yan, Yanzhe
Chu, Zhuangzhuang
Tang, Yi
Tang, Chunbo
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objectives.
Enhanced migration and osteogenic differentiation of mesenchymal stem cells (MSCs) are beneficial for MSC-mediated periodontal tissue regeneration, a promising method for periodontitis treatment.
FBXO5, a member of the F-box protein family, is involved in the osteogenic differentiation of MSCs.
Here, we investigated the effect of FBXO5 on human periodontal ligament stem cells (hPDLSCs).
Materials and Methods.
hPDLSCs were isolated from periodontal ligament tissue.
Lentivirus FBXO5 shRNA was used to silence FBXO5 expression.
Two transcripts of FBXO5 were overexpressed and transduced into hPDLSCs via retroviral infection.
Migration and osteogenic differentiation of hPDLSCs were evaluated using the scratch migration assay, alkaline phosphatase (ALP) activity, ALP staining, alizarin red staining, western blotting, and real-time polymerase chain reaction.
Results.
The expression of FBXO5 was upregulated after osteogenic induction in hPDLSCs.
FBXO5 knockdown attenuated migration, inhibited ALP activity and mineralization, and decreased RUNX2, OSX, and OCN expression, while the overexpression of two transcript isoforms significantly accelerated migration, enhanced ALP activity and mineralization, and increased RUNX2, OSX, and OCN expression in hPDLSCs.
Conclusions.
Both isoforms of FBXO5 promoted the migration and osteogenic differentiation potential of hPDLSCs, which identified a potential target for improving periodontal tissue regeneration.
American Psychological Association (APA)
Liu, Lin& Liu, Kun& Yan, Yanzhe& Chu, Zhuangzhuang& Tang, Yi& Tang, Chunbo. 2018. Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1128740
Modern Language Association (MLA)
Liu, Lin…[et al.]. Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1128740
American Medical Association (AMA)
Liu, Lin& Liu, Kun& Yan, Yanzhe& Chu, Zhuangzhuang& Tang, Yi& Tang, Chunbo. Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1128740
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128740