Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip
المؤلفون المشاركون
Zhang, Lifang
Wu, Ping
Pan, Qingjun
Lin, Jieping
Shi, Lei
Peng, Yanxia
Jiang, Na
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-02-25
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
This study was designed to prepare and evaluate the sensitivity and specificity of a Norovirus GI and GII fluorescent particles combined detection test strip method.
Using selected chromatographic materials and antibodies specific to Norovirus GI and GII, the Norovirus GI and GII fluorescent particles combined detection test strip (tested method) was prepared as a conventional double antibody sandwich.
The samples assayed included cultured rotavirus and 465 specimens from patients with symptoms of gastrointestinal infection.
Norovirus was detected using the tested method and a reference method (CerTest Norovirus GI-GII test card).
The results indicated that the sensitivity of the tested method was 4 (for GI detection) or 8 times (for GII detection) greater than the reference method.
Neither of the two methods cross-reacted with rotavirus and so on.
For specimens, 29 were found to be negative by the reference method and positive by the tested method, and 8 were found to be negative by the tested method and positive by the reference method.
Furthermore, a retesting of these samples by qPCR showed that 28 of the 29 were positive, and 3 of the 8 were positive.
In summary, the Norovirus GI and GII fluorescent particles combined detection test strip was successfully prepared and had good detection performance.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Lifang& Peng, Yanxia& Jiang, Na& Shi, Lei& Lin, Jieping& Wu, Ping…[et al.]. 2018. Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip. BioMed Research International،Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1128753
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Lifang…[et al.]. Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip. BioMed Research International No. 2018 (2018), pp.1-5.
https://search.emarefa.net/detail/BIM-1128753
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Lifang& Peng, Yanxia& Jiang, Na& Shi, Lei& Lin, Jieping& Wu, Ping…[et al.]. Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1128753
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128753
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر