Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip
Joint Authors
Zhang, Lifang
Wu, Ping
Pan, Qingjun
Lin, Jieping
Shi, Lei
Peng, Yanxia
Jiang, Na
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-25
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
This study was designed to prepare and evaluate the sensitivity and specificity of a Norovirus GI and GII fluorescent particles combined detection test strip method.
Using selected chromatographic materials and antibodies specific to Norovirus GI and GII, the Norovirus GI and GII fluorescent particles combined detection test strip (tested method) was prepared as a conventional double antibody sandwich.
The samples assayed included cultured rotavirus and 465 specimens from patients with symptoms of gastrointestinal infection.
Norovirus was detected using the tested method and a reference method (CerTest Norovirus GI-GII test card).
The results indicated that the sensitivity of the tested method was 4 (for GI detection) or 8 times (for GII detection) greater than the reference method.
Neither of the two methods cross-reacted with rotavirus and so on.
For specimens, 29 were found to be negative by the reference method and positive by the tested method, and 8 were found to be negative by the tested method and positive by the reference method.
Furthermore, a retesting of these samples by qPCR showed that 28 of the 29 were positive, and 3 of the 8 were positive.
In summary, the Norovirus GI and GII fluorescent particles combined detection test strip was successfully prepared and had good detection performance.
American Psychological Association (APA)
Zhang, Lifang& Peng, Yanxia& Jiang, Na& Shi, Lei& Lin, Jieping& Wu, Ping…[et al.]. 2018. Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip. BioMed Research International،Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1128753
Modern Language Association (MLA)
Zhang, Lifang…[et al.]. Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip. BioMed Research International No. 2018 (2018), pp.1-5.
https://search.emarefa.net/detail/BIM-1128753
American Medical Association (AMA)
Zhang, Lifang& Peng, Yanxia& Jiang, Na& Shi, Lei& Lin, Jieping& Wu, Ping…[et al.]. Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1128753
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128753