Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis
المؤلفون المشاركون
Yang, Guomin
Huang, Yinqiong
Wu, Xiaohong
Lin, Xiahong
Xu, Jinting
Chen, Xiaoyu
Bai, Xuefeng
Li, Qiulan
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-10
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Endogenous secretory receptor for advanced glycation end products (esRAGE) binds extracellular RAGE ligands and blocks RAGE activation on the cell surface, protecting endothelial cell function.
However, the underlying mechanism remains unclear.
Endothelial cells overexpressing the esRAGE gene were generated using a lentiviral vector.
Then, quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to assess esRAGE mRNA and protein levels, respectively.
Hoechst-PI double staining was used to assess apoptosis.
Western blot and qRT-PCR were used to assess the expression levels of apoptosis-related factors and the proinflammatory cytokine NF-кB.
Compared with the control group, AGEs significantly induced endothelial cell apoptosis, which was significantly reduced by esRAGE overexpression.
Incubation with AGEs upregulated the proapoptotic factor Bax and downregulated the antiapoptotic factor Bcl-2.
Overexpression of esRAGE reduced Bax expression induced by AGEs and increased Bcl-2 levels.
Furthermore, AGEs increased the expression levels of proinflammatory cytokine NF-кB, which were reduced after esRAGE overexpression.
esRAGE protects endothelial cells from AGEs associated apoptosis, by downregulating proapoptotic (Bax) and inflammatory (NF-кB) factors and upregulating the antiapoptotic factor Bcl-2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Guomin& Huang, Yinqiong& Wu, Xiaohong& Lin, Xiahong& Xu, Jinting& Chen, Xiaoyu…[et al.]. 2018. Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128985
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Guomin…[et al.]. Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1128985
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Guomin& Huang, Yinqiong& Wu, Xiaohong& Lin, Xiahong& Xu, Jinting& Chen, Xiaoyu…[et al.]. Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128985
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128985
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر