Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis
Joint Authors
Yang, Guomin
Huang, Yinqiong
Wu, Xiaohong
Lin, Xiahong
Xu, Jinting
Chen, Xiaoyu
Bai, Xuefeng
Li, Qiulan
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Endogenous secretory receptor for advanced glycation end products (esRAGE) binds extracellular RAGE ligands and blocks RAGE activation on the cell surface, protecting endothelial cell function.
However, the underlying mechanism remains unclear.
Endothelial cells overexpressing the esRAGE gene were generated using a lentiviral vector.
Then, quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to assess esRAGE mRNA and protein levels, respectively.
Hoechst-PI double staining was used to assess apoptosis.
Western blot and qRT-PCR were used to assess the expression levels of apoptosis-related factors and the proinflammatory cytokine NF-кB.
Compared with the control group, AGEs significantly induced endothelial cell apoptosis, which was significantly reduced by esRAGE overexpression.
Incubation with AGEs upregulated the proapoptotic factor Bax and downregulated the antiapoptotic factor Bcl-2.
Overexpression of esRAGE reduced Bax expression induced by AGEs and increased Bcl-2 levels.
Furthermore, AGEs increased the expression levels of proinflammatory cytokine NF-кB, which were reduced after esRAGE overexpression.
esRAGE protects endothelial cells from AGEs associated apoptosis, by downregulating proapoptotic (Bax) and inflammatory (NF-кB) factors and upregulating the antiapoptotic factor Bcl-2.
American Psychological Association (APA)
Yang, Guomin& Huang, Yinqiong& Wu, Xiaohong& Lin, Xiahong& Xu, Jinting& Chen, Xiaoyu…[et al.]. 2018. Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128985
Modern Language Association (MLA)
Yang, Guomin…[et al.]. Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1128985
American Medical Association (AMA)
Yang, Guomin& Huang, Yinqiong& Wu, Xiaohong& Lin, Xiahong& Xu, Jinting& Chen, Xiaoyu…[et al.]. Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128985
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128985