Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis

Joint Authors

Yang, Guomin
Huang, Yinqiong
Wu, Xiaohong
Lin, Xiahong
Xu, Jinting
Chen, Xiaoyu
Bai, Xuefeng
Li, Qiulan

Source

BioMed Research International

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-04-10

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Medicine

Abstract EN

Endogenous secretory receptor for advanced glycation end products (esRAGE) binds extracellular RAGE ligands and blocks RAGE activation on the cell surface, protecting endothelial cell function.

However, the underlying mechanism remains unclear.

Endothelial cells overexpressing the esRAGE gene were generated using a lentiviral vector.

Then, quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to assess esRAGE mRNA and protein levels, respectively.

Hoechst-PI double staining was used to assess apoptosis.

Western blot and qRT-PCR were used to assess the expression levels of apoptosis-related factors and the proinflammatory cytokine NF-кB.

Compared with the control group, AGEs significantly induced endothelial cell apoptosis, which was significantly reduced by esRAGE overexpression.

Incubation with AGEs upregulated the proapoptotic factor Bax and downregulated the antiapoptotic factor Bcl-2.

Overexpression of esRAGE reduced Bax expression induced by AGEs and increased Bcl-2 levels.

Furthermore, AGEs increased the expression levels of proinflammatory cytokine NF-кB, which were reduced after esRAGE overexpression.

esRAGE protects endothelial cells from AGEs associated apoptosis, by downregulating proapoptotic (Bax) and inflammatory (NF-кB) factors and upregulating the antiapoptotic factor Bcl-2.

American Psychological Association (APA)

Yang, Guomin& Huang, Yinqiong& Wu, Xiaohong& Lin, Xiahong& Xu, Jinting& Chen, Xiaoyu…[et al.]. 2018. Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128985

Modern Language Association (MLA)

Yang, Guomin…[et al.]. Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1128985

American Medical Association (AMA)

Yang, Guomin& Huang, Yinqiong& Wu, Xiaohong& Lin, Xiahong& Xu, Jinting& Chen, Xiaoyu…[et al.]. Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128985

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1128985