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Development of Quantitative Real-Time PCR Assays for Rapid and Sensitive Detection of Two Badnavirus Species in Sugarcane
المؤلفون المشاركون
Sun, Sheng-Ren
Fu, Hua-Ying
Gao, San-Ji
Ahmad, Kashif
Wu, Xiao-Bin
Chen, Jian-Sheng
Huang, Mei-Ting
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-06
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Sugarcane-infecting badnaviruses (sugarcane bacilliform viruses, SCBVs) represent a genetically heterogeneous species complex, posing a serious threat to the yield and quality of sugarcane in all major producing regions.
SCBVs are commonly transmitted across regions by the exchange of sugarcane germplasm.
In this study, we develop two quick, sensitive, and reliable protocols for real-time quantitative PCR (qPCR) of Sugarcane bacilliform MO virus (SCBMOV) and Sugarcane bacilliform IM virus (SCBIMV) using two sets of TaqMan probes and primers targeting the reverse transcriptase/ribonuclease H (RT/RNase H) region.
The two assays had a detection limit of 100 copies of plasmid DNA and were 100 times more sensitive than conventional PCR.
High specificity of the two assays was observed with respect to SCBIMV and SCBMOV.
A total of 176 sugarcane leaf tissue samples from Fujian and Yunnan provinces were collected and analyzed in parallel by conventional PCR, SCBIMV-qPCR, and SCBMOV-qPCR.
The SCBIMV-qPCR and SCBMOV-qPCR assays indicated that 50% (88/176) and 47% (83/176) samples tested positive, respectively, whereas only 29% (51/176) tested positive with conventional PCR with the primer pairs SCBV-F and SCBV-R.
We demonstrate for the first time that SCBIMV and SCBMOV occur in China and reveal coinfection of both Badnavirus species in 29% (51/176) of tested leaf samples.
Our findings supply sensitive and reliable qPCR assays for the detection and quantitation of SCBV in sugarcane quarantine programs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sun, Sheng-Ren& Ahmad, Kashif& Wu, Xiao-Bin& Chen, Jian-Sheng& Fu, Hua-Ying& Huang, Mei-Ting…[et al.]. 2018. Development of Quantitative Real-Time PCR Assays for Rapid and Sensitive Detection of Two Badnavirus Species in Sugarcane. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129284
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sun, Sheng-Ren…[et al.]. Development of Quantitative Real-Time PCR Assays for Rapid and Sensitive Detection of Two Badnavirus Species in Sugarcane. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1129284
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sun, Sheng-Ren& Ahmad, Kashif& Wu, Xiao-Bin& Chen, Jian-Sheng& Fu, Hua-Ying& Huang, Mei-Ting…[et al.]. Development of Quantitative Real-Time PCR Assays for Rapid and Sensitive Detection of Two Badnavirus Species in Sugarcane. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129284
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1129284
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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