![](/images/graphics-bg.png)
Development of Quantitative Real-Time PCR Assays for Rapid and Sensitive Detection of Two Badnavirus Species in Sugarcane
Joint Authors
Sun, Sheng-Ren
Fu, Hua-Ying
Gao, San-Ji
Ahmad, Kashif
Wu, Xiao-Bin
Chen, Jian-Sheng
Huang, Mei-Ting
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Sugarcane-infecting badnaviruses (sugarcane bacilliform viruses, SCBVs) represent a genetically heterogeneous species complex, posing a serious threat to the yield and quality of sugarcane in all major producing regions.
SCBVs are commonly transmitted across regions by the exchange of sugarcane germplasm.
In this study, we develop two quick, sensitive, and reliable protocols for real-time quantitative PCR (qPCR) of Sugarcane bacilliform MO virus (SCBMOV) and Sugarcane bacilliform IM virus (SCBIMV) using two sets of TaqMan probes and primers targeting the reverse transcriptase/ribonuclease H (RT/RNase H) region.
The two assays had a detection limit of 100 copies of plasmid DNA and were 100 times more sensitive than conventional PCR.
High specificity of the two assays was observed with respect to SCBIMV and SCBMOV.
A total of 176 sugarcane leaf tissue samples from Fujian and Yunnan provinces were collected and analyzed in parallel by conventional PCR, SCBIMV-qPCR, and SCBMOV-qPCR.
The SCBIMV-qPCR and SCBMOV-qPCR assays indicated that 50% (88/176) and 47% (83/176) samples tested positive, respectively, whereas only 29% (51/176) tested positive with conventional PCR with the primer pairs SCBV-F and SCBV-R.
We demonstrate for the first time that SCBIMV and SCBMOV occur in China and reveal coinfection of both Badnavirus species in 29% (51/176) of tested leaf samples.
Our findings supply sensitive and reliable qPCR assays for the detection and quantitation of SCBV in sugarcane quarantine programs.
American Psychological Association (APA)
Sun, Sheng-Ren& Ahmad, Kashif& Wu, Xiao-Bin& Chen, Jian-Sheng& Fu, Hua-Ying& Huang, Mei-Ting…[et al.]. 2018. Development of Quantitative Real-Time PCR Assays for Rapid and Sensitive Detection of Two Badnavirus Species in Sugarcane. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129284
Modern Language Association (MLA)
Sun, Sheng-Ren…[et al.]. Development of Quantitative Real-Time PCR Assays for Rapid and Sensitive Detection of Two Badnavirus Species in Sugarcane. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1129284
American Medical Association (AMA)
Sun, Sheng-Ren& Ahmad, Kashif& Wu, Xiao-Bin& Chen, Jian-Sheng& Fu, Hua-Ying& Huang, Mei-Ting…[et al.]. Development of Quantitative Real-Time PCR Assays for Rapid and Sensitive Detection of Two Badnavirus Species in Sugarcane. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129284
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129284