Inhibition of Acetylcholinesterase and Butyrylcholinesterase by a Plant Secondary Metabolite Boldine

المؤلفون المشاركون

Pohanka, Miroslav
Kostelnik, Adam

المصدر

BioMed Research International

العدد

المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-5، 5ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2018-04-05

دولة النشر

مصر

عدد الصفحات

5

التخصصات الرئيسية

الطب البشري

الملخص EN

Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are two enzymes sensitive to various chemical compounds having ability to bind to crucial parts of these enzymes.

Boldine is a natural alkaloid and it was mentioned in some older works that it can inhibit some kinds of AChE.

We reinvestigated this effect on AChE and also on BChE using acetyl (butyryl) thiocholine and Ellman’s reagents as standard substances for spectrophotometric assay.

We found out IC50 of AChE equal to 372 μmol/l and a similar level to BChE, 321 μmol/l.

We conclude our experiment by a finding that boldine is cholinesterase inhibitor; however we report significantly weaker inhibition than that suggested in literature.

Likewise, we tried to investigate the mechanism of inhibition and completed it with in silico study.

Potential toxic effect on cholinesterases in real conditions is also discussed.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Kostelnik, Adam& Pohanka, Miroslav. 2018. Inhibition of Acetylcholinesterase and Butyrylcholinesterase by a Plant Secondary Metabolite Boldine. BioMed Research International،Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1129923

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Kostelnik, Adam& Pohanka, Miroslav. Inhibition of Acetylcholinesterase and Butyrylcholinesterase by a Plant Secondary Metabolite Boldine. BioMed Research International No. 2018 (2018), pp.1-5.
https://search.emarefa.net/detail/BIM-1129923

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Kostelnik, Adam& Pohanka, Miroslav. Inhibition of Acetylcholinesterase and Butyrylcholinesterase by a Plant Secondary Metabolite Boldine. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1129923

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1129923