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Inhibition of Acetylcholinesterase and Butyrylcholinesterase by a Plant Secondary Metabolite Boldine
Joint Authors
Pohanka, Miroslav
Kostelnik, Adam
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-05
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are two enzymes sensitive to various chemical compounds having ability to bind to crucial parts of these enzymes.
Boldine is a natural alkaloid and it was mentioned in some older works that it can inhibit some kinds of AChE.
We reinvestigated this effect on AChE and also on BChE using acetyl (butyryl) thiocholine and Ellman’s reagents as standard substances for spectrophotometric assay.
We found out IC50 of AChE equal to 372 μmol/l and a similar level to BChE, 321 μmol/l.
We conclude our experiment by a finding that boldine is cholinesterase inhibitor; however we report significantly weaker inhibition than that suggested in literature.
Likewise, we tried to investigate the mechanism of inhibition and completed it with in silico study.
Potential toxic effect on cholinesterases in real conditions is also discussed.
American Psychological Association (APA)
Kostelnik, Adam& Pohanka, Miroslav. 2018. Inhibition of Acetylcholinesterase and Butyrylcholinesterase by a Plant Secondary Metabolite Boldine. BioMed Research International،Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1129923
Modern Language Association (MLA)
Kostelnik, Adam& Pohanka, Miroslav. Inhibition of Acetylcholinesterase and Butyrylcholinesterase by a Plant Secondary Metabolite Boldine. BioMed Research International No. 2018 (2018), pp.1-5.
https://search.emarefa.net/detail/BIM-1129923
American Medical Association (AMA)
Kostelnik, Adam& Pohanka, Miroslav. Inhibition of Acetylcholinesterase and Butyrylcholinesterase by a Plant Secondary Metabolite Boldine. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1129923
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129923