Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves
المؤلفون المشاركون
Moura, Sidnei
Bruck de Souza, Letiele
Leitão Gindri, Amanda
de Andrade Fortes, Thainara
Felli Kubiça, Thais
Enderle, Jefferson
Roehrs, Rafael
Manfredini, Vanusa
Gasparotto Denardin, Elton Luís
المصدر
Advances in Pharmacological and Pharmaceutical Sciences
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-01
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Context.
Chaptalia nutans (L.) Pol.
(family: Asteraceae) is widely used in traditional medicine as laxative and anticough medications and especially in the traumatisms, wounds, and hemorrhages in topical preparations.
Objective.
This work was to evaluate the chemical constitution of the hydromethanolic (30/70 methanol-water) macerating extract obtained from the leaves of C.
nutans, as well as to study the antioxidant, antimicrobial, cytotoxic, and genotoxic activity of the species.
Materials and methods.
Phytochemical screening, antioxidant activity (total phenolic, total flavonoid, condensed tannins content, DPPH radical, and FRAP), antibacterial activity (P.
aeruginosa, B.
cereus, E.
epidermidis, E.
coli, S.
aureus, E.
faecalis, P.
mirabilis, Candida glabrata (clinical isolate), Candida tropicalis (clinical isolate), C.
krusei (clinical isolate), and C.
albicans (clinical isolate)), and oxidative stress parameters (TBARS, carbonyl protein, and DCFH) were analyzed according to the literature.
Toxicity of C.
nutans was evaluated using an alternative method, D.
melanogaster, as well as a locomotor assay.
Results.
The phytochemical screening test of methanolic leaves extract revealed the presence of alkaloids, coumarins, quaternary bases, phenolics, flavonoids, tannins, and free steroids.
A quantitative phytochemical study indicated the total phenol (30.17 ± 1.44 mg/g), flavonoid (21.64 ± 0.66 mg/g), and condensed tannins (9.58 ± 0.99 mg/g).
DPPH (345.41 ± 5.35 μg/mL) and FRAP (379.98 ± 39.25 μM FeSO4/mg sample) show to extract of C.
nutans leaves an intermediate value, indicating moderate antioxidant activity of the extract.
Antibacterial results revealed only a positive result (antimicrobial activity) for the hexane fraction which significantly inhibited the microorganisms E.
epidermidis, C.
tropicalis, C.
glabrata, and C.
krusei at a concentration of 1000 μg/mL.
TBARS, carbonyl protein, and DCFH demonstrate that the extract has the ability to protect the cell from protein and lipid damage, as well as the inhibition of oxygen-derived radicals at the three concentrations tested: 0.1, 1, and 10 mg/mL.
Regarding the toxicity of C.
nutans extract against D.
melanogaster, it was found that until the concentration of 15 mg/mL, the extract showed no toxicity and that the LC50 obtained was 24 mg/mL.
Results show that the C.
nutans extract leaves used to prevent PQ damage were effective in reducing flies’ mortality and improving locomotor capacity.
Conclusion.
Our studies demonstrated for the first time that C.
nutans crude leaf extract has high antioxidant capacity both in vitro and in vivo through different analysis techniques.
These results make it possible to infer future applications in the pharmacological area, evidenced by the low toxicity observed in D.
melanogatser, as well as the ability to neutralize different sources of RONS.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bruck de Souza, Letiele& Leitão Gindri, Amanda& de Andrade Fortes, Thainara& Felli Kubiça, Thais& Enderle, Jefferson& Roehrs, Rafael…[et al.]. 2020. Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves. Advances in Pharmacological and Pharmaceutical Sciences،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1130082
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bruck de Souza, Letiele…[et al.]. Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves. Advances in Pharmacological and Pharmaceutical Sciences No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1130082
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bruck de Souza, Letiele& Leitão Gindri, Amanda& de Andrade Fortes, Thainara& Felli Kubiça, Thais& Enderle, Jefferson& Roehrs, Rafael…[et al.]. Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves. Advances in Pharmacological and Pharmaceutical Sciences. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1130082
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130082
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر