Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves

Joint Authors

Moura, Sidnei
Bruck de Souza, Letiele
Leitão Gindri, Amanda
de Andrade Fortes, Thainara
Felli Kubiça, Thais
Enderle, Jefferson
Roehrs, Rafael
Manfredini, Vanusa
Gasparotto Denardin, Elton Luís

Source

Advances in Pharmacological and Pharmaceutical Sciences

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-05-01

Country of Publication

Egypt

No. of Pages

15

Main Subjects

Pharmacology

Abstract EN

Context.

Chaptalia nutans (L.) Pol.

(family: Asteraceae) is widely used in traditional medicine as laxative and anticough medications and especially in the traumatisms, wounds, and hemorrhages in topical preparations.

Objective.

This work was to evaluate the chemical constitution of the hydromethanolic (30/70 methanol-water) macerating extract obtained from the leaves of C.

nutans, as well as to study the antioxidant, antimicrobial, cytotoxic, and genotoxic activity of the species.

Materials and methods.

Phytochemical screening, antioxidant activity (total phenolic, total flavonoid, condensed tannins content, DPPH radical, and FRAP), antibacterial activity (P.

aeruginosa, B.

cereus, E.

epidermidis, E.

coli, S.

aureus, E.

faecalis, P.

mirabilis, Candida glabrata (clinical isolate), Candida tropicalis (clinical isolate), C.

krusei (clinical isolate), and C.

albicans (clinical isolate)), and oxidative stress parameters (TBARS, carbonyl protein, and DCFH) were analyzed according to the literature.

Toxicity of C.

nutans was evaluated using an alternative method, D.

melanogaster, as well as a locomotor assay.

Results.

The phytochemical screening test of methanolic leaves extract revealed the presence of alkaloids, coumarins, quaternary bases, phenolics, flavonoids, tannins, and free steroids.

A quantitative phytochemical study indicated the total phenol (30.17 ± 1.44 mg/g), flavonoid (21.64 ± 0.66 mg/g), and condensed tannins (9.58 ± 0.99 mg/g).

DPPH (345.41 ± 5.35 μg/mL) and FRAP (379.98 ± 39.25 μM FeSO4/mg sample) show to extract of C.

nutans leaves an intermediate value, indicating moderate antioxidant activity of the extract.

Antibacterial results revealed only a positive result (antimicrobial activity) for the hexane fraction which significantly inhibited the microorganisms E.

epidermidis, C.

tropicalis, C.

glabrata, and C.

krusei at a concentration of 1000 μg/mL.

TBARS, carbonyl protein, and DCFH demonstrate that the extract has the ability to protect the cell from protein and lipid damage, as well as the inhibition of oxygen-derived radicals at the three concentrations tested: 0.1, 1, and 10 mg/mL.

Regarding the toxicity of C.

nutans extract against D.

melanogaster, it was found that until the concentration of 15 mg/mL, the extract showed no toxicity and that the LC50 obtained was 24 mg/mL.

Results show that the C.

nutans extract leaves used to prevent PQ damage were effective in reducing flies’ mortality and improving locomotor capacity.

Conclusion.

Our studies demonstrated for the first time that C.

nutans crude leaf extract has high antioxidant capacity both in vitro and in vivo through different analysis techniques.

These results make it possible to infer future applications in the pharmacological area, evidenced by the low toxicity observed in D.

melanogatser, as well as the ability to neutralize different sources of RONS.

American Psychological Association (APA)

Bruck de Souza, Letiele& Leitão Gindri, Amanda& de Andrade Fortes, Thainara& Felli Kubiça, Thais& Enderle, Jefferson& Roehrs, Rafael…[et al.]. 2020. Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves. Advances in Pharmacological and Pharmaceutical Sciences،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1130082

Modern Language Association (MLA)

Bruck de Souza, Letiele…[et al.]. Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves. Advances in Pharmacological and Pharmaceutical Sciences No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1130082

American Medical Association (AMA)

Bruck de Souza, Letiele& Leitão Gindri, Amanda& de Andrade Fortes, Thainara& Felli Kubiça, Thais& Enderle, Jefferson& Roehrs, Rafael…[et al.]. Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves. Advances in Pharmacological and Pharmaceutical Sciences. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1130082

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1130082