Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection
المؤلفون المشاركون
Rodriguez-Padilla, Cristina
Trejo-Chávez, Armando
de la Garza-Ramos, Myriam Angelica
Caballero-Hernandez, Diana
Tamez-Guerra, Reyes Silvestre
Gomez-Flores, Ricardo
Tamez-Guerra, Patricia
Espinosa-Ramos, David
Pérez-Limón, Luis Jerónimo
المصدر
Canadian Journal of Infectious Diseases and Medical Microbiology
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-01
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Helicobacter pylori is a spiral Gram-negative bacterium associated with inflammation of the gastric mucosa, peptic ulcer, and gastric adenocarcinoma, whose treatment has failed due to antibiotic resistance and side effects.
Furthermore, because there are no vaccines effective against H.
pylori, an appropriate vaccine design targeting conserved/essential genes must be identified.
In the present study, a H.
pylori 50–52 kDa immunogen-derived peptide antigen with the sequence Met-Val-Thr-Leu-Ile-Asn-Asn-Glu (MVTLINNE) was used to immunize against H.
pylori infection.
For this, mice received an intraperitoneal injection of 100 μg of H.
pylori peptide on the first week, followed by two weekly subcutaneous reinforcements and further 109 bacteria administration in the drinking water for 3 weeks.
Thymic cells proliferative responses to concanavalin A, serum levels of IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ, and TNF-α cytokines, and IgG1, IgG2a, IgG2b, IgG3 IgM, and IgA immunoglobulins were evaluated.
Significant (p<0.05) increases on lymphoproliferation and spleen weights after immunization were observed.
In contrast, infection significantly (p<0.05) decreased lymphoproliferation, which was recovered in immunized mice.
In addition, levels of serum TH1 and TH2 cytokines were not altered after immunization, except for the significant increase in IL-6 production in immunized and/or infected animals.
Moreover, immunization correlated with plasma secretory IgA and IgG, whereas infection alone only elicited IgM antibodies.
Peptide immunization protected 100% of mice against virulent H.
pylori.
MVTLINNE peptide deserves further research as an approach to the prophylaxis of H.
pylori infection.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Espinosa-Ramos, David& Caballero-Hernandez, Diana& Gomez-Flores, Ricardo& Trejo-Chávez, Armando& Pérez-Limón, Luis Jerónimo& de la Garza-Ramos, Myriam Angelica…[et al.]. 2019. Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1130117
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Espinosa-Ramos, David…[et al.]. Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1130117
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Espinosa-Ramos, David& Caballero-Hernandez, Diana& Gomez-Flores, Ricardo& Trejo-Chávez, Armando& Pérez-Limón, Luis Jerónimo& de la Garza-Ramos, Myriam Angelica…[et al.]. Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection. Canadian Journal of Infectious Diseases and Medical Microbiology. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1130117
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130117
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر