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Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection
Joint Authors
Rodriguez-Padilla, Cristina
Trejo-Chávez, Armando
de la Garza-Ramos, Myriam Angelica
Caballero-Hernandez, Diana
Tamez-Guerra, Reyes Silvestre
Gomez-Flores, Ricardo
Tamez-Guerra, Patricia
Espinosa-Ramos, David
Pérez-Limón, Luis Jerónimo
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Helicobacter pylori is a spiral Gram-negative bacterium associated with inflammation of the gastric mucosa, peptic ulcer, and gastric adenocarcinoma, whose treatment has failed due to antibiotic resistance and side effects.
Furthermore, because there are no vaccines effective against H.
pylori, an appropriate vaccine design targeting conserved/essential genes must be identified.
In the present study, a H.
pylori 50–52 kDa immunogen-derived peptide antigen with the sequence Met-Val-Thr-Leu-Ile-Asn-Asn-Glu (MVTLINNE) was used to immunize against H.
pylori infection.
For this, mice received an intraperitoneal injection of 100 μg of H.
pylori peptide on the first week, followed by two weekly subcutaneous reinforcements and further 109 bacteria administration in the drinking water for 3 weeks.
Thymic cells proliferative responses to concanavalin A, serum levels of IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ, and TNF-α cytokines, and IgG1, IgG2a, IgG2b, IgG3 IgM, and IgA immunoglobulins were evaluated.
Significant (p<0.05) increases on lymphoproliferation and spleen weights after immunization were observed.
In contrast, infection significantly (p<0.05) decreased lymphoproliferation, which was recovered in immunized mice.
In addition, levels of serum TH1 and TH2 cytokines were not altered after immunization, except for the significant increase in IL-6 production in immunized and/or infected animals.
Moreover, immunization correlated with plasma secretory IgA and IgG, whereas infection alone only elicited IgM antibodies.
Peptide immunization protected 100% of mice against virulent H.
pylori.
MVTLINNE peptide deserves further research as an approach to the prophylaxis of H.
pylori infection.
American Psychological Association (APA)
Espinosa-Ramos, David& Caballero-Hernandez, Diana& Gomez-Flores, Ricardo& Trejo-Chávez, Armando& Pérez-Limón, Luis Jerónimo& de la Garza-Ramos, Myriam Angelica…[et al.]. 2019. Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1130117
Modern Language Association (MLA)
Espinosa-Ramos, David…[et al.]. Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1130117
American Medical Association (AMA)
Espinosa-Ramos, David& Caballero-Hernandez, Diana& Gomez-Flores, Ricardo& Trejo-Chávez, Armando& Pérez-Limón, Luis Jerónimo& de la Garza-Ramos, Myriam Angelica…[et al.]. Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection. Canadian Journal of Infectious Diseases and Medical Microbiology. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1130117
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130117