Application of kDNA Minicircle PCR-RFLP to Characterize Leishmania donovani Clinical Isolates Obtained from Post-Kala-Azar Dermal Leishmaniasis in Eastern Nepal
المؤلفون المشاركون
Rijal, Suman
Khanal, Basudha
Rai, Keshav
Bhattarai, Narayan Raj
Pokhrel, Ojesh
Rijal, Arpana
المصدر
Canadian Journal of Infectious Diseases and Medical Microbiology
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-07-30
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Post-kala-azar dermal leishmaniasis (PKDL) is a skin manifestation of visceral leishmaniasis (VL) which develops after apparent cure in some patients.
PKDL is considered as the potential reservoir for the VL infection.
Molecular epidemiological characterization of L.
donovani isolates obtained from VL and PKDL isolates is essentially required in order to understand the transmission dynamics of the VL infection.
To date, genetic variation among the VL and PKDL L.
donovani isolates was not fully elucidated.
Therefore, 14 clinical isolates from VL and 4 clinical isolates from PKDL were speciated by hsp70 and rDNA genes.
Further characterization of L.
donovani by haspB PCR demonstrates two different genotypes.
All PKDL isolates have the same genetic structure.
kDNA PCR-RFLP assay revealed 18 different genotypes; however, structural analysis showed the two distinct kDNA genotype population (k = 2).
The kDNA fingerprint patterns of parasites from hilly districts were clustered separately from low-land districts.
Therefore, further study with a large number of samples is urgently required for systematic characterization of the clinical isolates to track the molecular epidemiology of the Leishmania donovani causing VL and the role of PKDL as a reservoir.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pokhrel, Ojesh& Rai, Keshav& Bhattarai, Narayan Raj& Rijal, Suman& Rijal, Arpana& Khanal, Basudha. 2019. Application of kDNA Minicircle PCR-RFLP to Characterize Leishmania donovani Clinical Isolates Obtained from Post-Kala-Azar Dermal Leishmaniasis in Eastern Nepal. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1130133
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pokhrel, Ojesh…[et al.]. Application of kDNA Minicircle PCR-RFLP to Characterize Leishmania donovani Clinical Isolates Obtained from Post-Kala-Azar Dermal Leishmaniasis in Eastern Nepal. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1130133
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pokhrel, Ojesh& Rai, Keshav& Bhattarai, Narayan Raj& Rijal, Suman& Rijal, Arpana& Khanal, Basudha. Application of kDNA Minicircle PCR-RFLP to Characterize Leishmania donovani Clinical Isolates Obtained from Post-Kala-Azar Dermal Leishmaniasis in Eastern Nepal. Canadian Journal of Infectious Diseases and Medical Microbiology. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1130133
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130133
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر