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Application of kDNA Minicircle PCR-RFLP to Characterize Leishmania donovani Clinical Isolates Obtained from Post-Kala-Azar Dermal Leishmaniasis in Eastern Nepal
Joint Authors
Rijal, Suman
Khanal, Basudha
Rai, Keshav
Bhattarai, Narayan Raj
Pokhrel, Ojesh
Rijal, Arpana
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Post-kala-azar dermal leishmaniasis (PKDL) is a skin manifestation of visceral leishmaniasis (VL) which develops after apparent cure in some patients.
PKDL is considered as the potential reservoir for the VL infection.
Molecular epidemiological characterization of L.
donovani isolates obtained from VL and PKDL isolates is essentially required in order to understand the transmission dynamics of the VL infection.
To date, genetic variation among the VL and PKDL L.
donovani isolates was not fully elucidated.
Therefore, 14 clinical isolates from VL and 4 clinical isolates from PKDL were speciated by hsp70 and rDNA genes.
Further characterization of L.
donovani by haspB PCR demonstrates two different genotypes.
All PKDL isolates have the same genetic structure.
kDNA PCR-RFLP assay revealed 18 different genotypes; however, structural analysis showed the two distinct kDNA genotype population (k = 2).
The kDNA fingerprint patterns of parasites from hilly districts were clustered separately from low-land districts.
Therefore, further study with a large number of samples is urgently required for systematic characterization of the clinical isolates to track the molecular epidemiology of the Leishmania donovani causing VL and the role of PKDL as a reservoir.
American Psychological Association (APA)
Pokhrel, Ojesh& Rai, Keshav& Bhattarai, Narayan Raj& Rijal, Suman& Rijal, Arpana& Khanal, Basudha. 2019. Application of kDNA Minicircle PCR-RFLP to Characterize Leishmania donovani Clinical Isolates Obtained from Post-Kala-Azar Dermal Leishmaniasis in Eastern Nepal. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1130133
Modern Language Association (MLA)
Pokhrel, Ojesh…[et al.]. Application of kDNA Minicircle PCR-RFLP to Characterize Leishmania donovani Clinical Isolates Obtained from Post-Kala-Azar Dermal Leishmaniasis in Eastern Nepal. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1130133
American Medical Association (AMA)
Pokhrel, Ojesh& Rai, Keshav& Bhattarai, Narayan Raj& Rijal, Suman& Rijal, Arpana& Khanal, Basudha. Application of kDNA Minicircle PCR-RFLP to Characterize Leishmania donovani Clinical Isolates Obtained from Post-Kala-Azar Dermal Leishmaniasis in Eastern Nepal. Canadian Journal of Infectious Diseases and Medical Microbiology. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1130133
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130133